摘要
目的：为了解丙型肝炎患者血清HCVRNA含量与疾病程度及血清丙氨酸转氨酶（ALT）水平的关系。方法：采用荧光聚合酶健反应（PCR）的方法，对105例不同临床类型丙型肝炎患者的血清HCVRNA进行定量检测。结果：丙型肝炎血清HCVRNA含量在103～109．5拷贝／ml之间。无症状HCV感染者血清HCVRNA含量（105.2±1.8拷贝／ml）显著低于急性丙型肝炎患者（107.5±2.4拷贝／ml）、慢性丙型肝炎患者（107.8±3.1拷贝／ml）及肝硬化（107.6±2.5拷贝／ml）。相关分析显示，慢性丙型肝炎病毒血症水平与血清ALT呈显著正相关。结论：高水平的HCV复制可能在肝损害和肝脏疾病的进展中发挥着重要作用。
Objective:In order to understand the relationship among the relationship among the amount of HCV RNA, severity of liver disease and serum ALT levels. Methods: A quantitative PCR is described for serum HCV RNA which is based on a Amplisensor assay.Results: The results showed that the amounts of HCV RNA varied from 103 to 109.5 copies/ml of serum in 105 patients with hepatitis C.The serum HCV RNA levds of asymptomic HCV carriers(105.2±1. 8 copies/ml) were significantly lower than that of acute hepatitis(107.5±2.4 copies/ml), chronic hepatitis(107.8±3.1 copies/ml) and liver cirrhosis(107.6±2.5 copies/ml). The concentrations of HCV RNA correlated positively with the serum ALT levels in chronic hepatitis C.Conclusions: The result suggested that the replicative level of hepatitis C virus is higher in acute hepatitis and advanced liver disease and the mechanism leading to the HCV - induced hepatocyte lesion is of importance in the management of HCV - infected patients.
出处
《广东医学》
CAS
CSCD
1998年第9期658-659,共2页
Guangdong Medical Journal
基金
广东省重点攻关项目!94062
广州市医药卫生青年基金!95004
