摘要
目的探讨放射性损伤骨髓衰竭的机理及防护对策。方法用Ｎｏｒｔｈｅｒｎｂｌｏｔ技术检测了６０Ｃｏγ射线８．０Ｇｙ照射小鼠（对照组）及照射后立即给复方活血汤治疗小鼠（中药组）的骨髓有核细胞ｂｃｌ－２、ｂａｘ、ｂｃｌ－ｘＬ基因表达。结果放射损伤后第３，７天，对照组骨髓有核细胞ｂｃｌ－２／ｂａｘ值减小、ｂｃｌ－ｘＬ不表达。中药组与之相反，ｂｃｌ－２／ｂａｘ值明显增大、ｂｃｌ－ｘＬ明显表达。受照后第７天两组脾脏单个核细胞ｂｃｌ－２／ｂａｘ值与骨髓有核细胞的表达一致。结论复方活血汤对放射损伤骨髓造血细胞增殖周期调控基因ｂｃｌ－２、ｂａｘ、ｂｃｌ－ｘＬ有一定调节作用。
Objective\ To explore the mechanism of and protective strategy againt bone marrow failure caused by radiation injury.Method\ Northern blot technique was used to detect the bcl 2,bax and bcl x L gene expression in murine bone marrow nucleated cells (BMNC) both in Chinese drug treated group which was given per os compound blood activating decoction immediately after irradiation with 8.0 Gy60Co γ rays,and in control group.Results\ On the 3rd and 7th day after irradiation,bcl 2/bax value of BMNC decreased obviously.No bcl x L expression was observed in control group.In Chinese drug treated group,the bcl 2/bax value increased remarkably,while bcl x L expression occurred obviously.On the 7th day after irradiation,the bcl 2/bax value in splenic mononuclear cells was consitstent with that in BMNC in both Chinese drug treated and control groups.Conclusion\ Compound blood activating decoction could have a regulative effect on the expression of cell cycle regulation gene bcl 2,bax and bcl xL in irradiated bone marrow hematopoietic cells and protect the bone marrow hematopoiesis.
出处
《中华放射医学与防护杂志》
CAS
CSCD
北大核心
1998年第4期256-258,共3页
Chinese Journal of Radiological Medicine and Protection
基金
国家自然科学基金
关键词
放射损伤
造血细胞
基因表达
复方活血汤
Radiation injury\ \ Hematopoietic cells\ \ bcl 2 gene\ \ bax gene\ \ bcl x L gene
