摘要
目的:寻找一种有效纯化连钱草总黄酮的方法并对工艺条件进行初步摸索。方法:以连钱草总黄酮洗脱率和纯度为考察指标,确定大孔树脂分离纯化连钱草总黄酮的吸附和洗脱参数。结果:药液浓度(总黄酮浓度0.383mg/mL),上样量20mL,以1.0 mL/min的速度进行吸附,5 BV纯化水、10 BV 30%乙醇依次以1.0 mL/min的速度进行洗脱效果最佳,醇洗脱液浓缩后总干燥物中连钱草总黄酮的含量达68.94%。结论:该方法对连钱草总黄酮有较好的纯化作用,且工艺简单,成本低,具有良好的应用前景。
Objective：To study the purification process of total flavones from Glechoma longituba with macroporous adsorption resin. Methods：The eluting power and purified ratio of total flavones were selected as indices,the adsorbility and eluting parameters of the process were studied.Results：The best result that the purity of total flavones from Glechoma longituba could reach 68.94%was based on the followings：total flavones content in the liquid-0.383 mg/mL,feeding volume-20mL,feeding rate-1.0 mL/min,eluting agent -5BV purify water and 10BV 30%ethanol,eluting rate-1.0 mL/min.Conclution：The process is simple and convenient which can be used in the purification of total flavones from Glechoma longituba,it may has a good application foreground.
出处
《中药材》
CAS
CSCD
北大核心
2009年第12期1894-1898,共5页
Journal of Chinese Medicinal Materials
关键词
大孔吸附树脂
连钱草总黄酮
分离纯化
Macroporous adsorption resin
Total flavones of Glechoma longituba
Separation and purification
