摘要
采用Tris-HCl法提取泥鳅抗菌肽,SephadexG-50凝胶过滤层析分离泥鳅抗菌肽,并通过微量反映法测定泥鳅抗菌肽对株嗜水气单胞菌、大肠杆菌和金黄色葡萄球菌的最低抑菌浓度(MIC)。结果表明:泥鳅抗菌肽对株嗜水气单胞菌、大肠杆菌和金黄色葡萄球菌的MIC分别为0.195、3.125、12.50μg/μL,具有较好的抗菌活性。
Using Tris-HCl to extract the loach cecropin and SephadexG-50 to filtration chromatograhpy the MIC of loach cecropin to the Aeromonas hydrophila,Escherichia coli and Staphylococcusaureus were reflected through the micro-determination.The results showed as follows：the MIC of the loach cecropin to the Aeromonas hydrophila,Escherichia coli and Staphylococcusaureus were 0.195,3.125,12.5μg/μL,which had the better antibacterial activity.
出处
《现代农业科技》
2009年第24期296-297,共2页
Modern Agricultural Science and Technology
基金
吉林省重大科技攻关项目(吉科20060208)
关键词
泥鳅
抗菌肽
最低抑菌浓度
loach
cecropin
minimum inhibition concentration（MIC）
