摘要
目的观察寿胎丸对反复自然流产小鼠母胎界面CD4+ T细胞STAT信号转导途径的影响。方法采用CBA/J×BALB/C建立正常妊娠小鼠模型,CBA/J×DBA/2建立反复自然流产小鼠模型。流产模型小鼠随机分为对照组及寿胎丸组,给药14d后取其蜕膜和胎盘组织,分离CD4+ T细胞,Springbio720点抗体芯片检测STAT信号转导途径蛋白磷酸化水平。结果寿胎丸可上调STAT信号转导途径的STAT3和STAT6蛋白的磷酸化水平,同时下调STAT1蛋白的磷酸化水平。结论STAT3和STAT6信号转导途径的激活可能是寿胎丸治疗反复自然流产的重要分子机制之一。
Objective To study the effects of Shoutaiwan on the STAT signal transduction pathway of CD4^＋ T cells in maternal-fetal interface of mice with recurrent spontaneous abortion （RSA）. Methods CBA/J× BALB/C mating combination and CBA/J× DBA/2 mating combination were used as normal pregnancy and RSA model respectively. Mice with RSA were randomly assigned to model group and Shoutaiwan group. After 14 days of administration, the STAT signal transduction pathway of CD4^＋ T cells in mice decidual and placental tissues were detected by Springbio 720 antibody microarrays. Results Shoutalwan could increase the phosphorylation level of STAT3 and STAT6 protein in the STAT signal transduction pathway. At the same time, Shoutaiwan could decrease the phosphorylation level of STAT1 protein. Conclusion The activation of STAT3 and STAT6 signal transduction pathway might play a important role in the therapy of RSA by Shoutalwan.
出处
《中国中医药信息杂志》
CAS
CSCD
2010年第1期23-25,共3页
Chinese Journal of Information on Traditional Chinese Medicine
基金
湖南省科技计划重点资助项目(2007FJ2005)
关键词
寿胎丸
反复自然流产
信号转导
小鼠
Shoutalwan： recurrent spontaneous abortion： signal transduction
mice
