摘要
通直型巨龙竹Dendrocalamus sinicus是云南重要的经济竹种资源之一。为了保护和开发通直型巨龙竹种质资源,应用简单序列重复区间扩增(ISSR)标记对通直型巨龙竹核心分布区不同地理种源的遗传变异进行了研究。从80个引物中筛选出7个用于正式扩增,在所研究的4个居群共54个个体中检测到54个多态位点。在居群水平上,多态位点百分率为9.59%,Nei’s基因多样性指数(H)和Shannon信息指数(I)分别为0.036 3和0.053 6;在物种水平上,多态位点百分率为73.97%,H和I分别为0.260 0和0.392 1。居群间的遗传分化系数(Gst)达0.863 4,显示不同居群间遗传分化很大。造成通直型巨龙竹不同地理种源遗传分化的主要原因可能是生境片断化和自然条件下花而不实造成的种子传播困难。
The straight-culmed type of Dendrocalamus sinicus is one of the most important economic bamboo resources in Yunnan Province, China. To protect and develop native germplasm resources of the straight-culmed D. sinicus in Yunnan, the genetic diversity of four straight-culmed provenances of D. sinicus was analyzed based on inter-simple sequence repeat（ISSR） markers for percentage of polymorphic bands （PPB）, Nei＇s gene diversity（H）, Shannon＇s information index（I） and Nei＇s coefficient of genetic differentiation（Gst）. Results showed seven informative and reliable primers with 54 polymorphic loci that were chosen from 80 pre-screened primers. At the population level, PPB was 9.59%, H was 0.036 3, and I was 0.053 6; whereas at the species level, PPB was 73.97%, H was 0.260 0 and I was 0.3921. Also, G, was 0.863 4. G, indicated a high degree of genetic differentiation among straight-culmed D. sinicus provenances most likely due to habitat fragmentation and flowering without seed. [Ch, 4 fig. 4 tab. 21 ref.]
出处
《浙江林学院学报》
CSCD
北大核心
2010年第1期81-86,共6页
Journal of Zhejiang Forestry College
基金
"十一五"国家科技支撑计划项目(2006BAD19B0301)
国际竹藤网络中心基本科研业务费专项资金项目(06/07-D37)
云南省科学技术厅联合支持国家科技项目(2007GA014)
关键词
林木育种学
巨龙竹
遗传分化
简单序列重复区间扩增
forest tree breeding
Dendrocalamus sinicus
genetic differentiation
inter-simple sequence repeat （ISSR）
