摘要
目的观察瑞芬太尼预处理对脑缺血/再灌注(I/R)大鼠丙二醛(MDA)含量和超氧化物歧化酶(SOD)活性的影响,以探讨其脑保护作用的机制。方法成年Wistar大鼠54只随机分为3组:假手术(Sham)组、缺血/再灌注(IR)组和瑞芬太尼预处理(Rem)组,每组18只。采用大鼠4条血管阻断方法制备大鼠全脑I/R模型,各组在大鼠脑缺血15min后,设再灌注6、12、24h组,相应时间点检测各组脑组织MDA含量和SOD活性,光镜下观察脑组织病理变化。结果与Sham组比较,IR组MDA含量明显升高,SOD活性明显降低(P均<0.05),与IR组比较,Rem组可显著减少大鼠脑I/R损伤后MDA含量,提高SOD活性(P均<0.05)。结论瑞芬太尼预处理对大鼠脑I/R损伤有一定的保护作用,其机制可能与抑制自由基损伤有关。
Objective To observe the effects of remifentanil pretreatment on the brain tissue malondialdehyde（MDA）concentration and superoxide dismutase（SOD）activity in rats induced by cerebral ischemia-reperfusion（CIR） so as to investigate cerebral protection mechanism of remifentanil. Methods A total of 54 adult male Wistar rats were randomly divided into three groups（n=18 each）： sham operation group（Sham）, ischemia-reperfusion group （IR）, and remifentanil pretreatment group（Rem）. Four-vessel occlusion was used to establish the global cerebral ischemia reperfusion model in the study groups .An acute experimental cerebral ischemia rats model induced for 15 minutes, all groups were further divided into 3 subgroups according to the reperfusion time （6,12 and 24 h） to test the brain tissue MDA concentration and SOD activity, to observe the morphological changes of rat cerebral by optical microscope. Results Compared with the Sham group, the MDA concentration in the rat brain tissue significantly increased in IR group（P〈0.05）, SOD activity significantly decreased （P〈0.05）; Compared with the IR group, the MDA concentration in the rat brain tissue in Rem group remarkably decreased （all P〈0.05）, while the SOD activity obviously increased （all P〈0.05）. Conclusion Remifentanil pretreatment shows an neuroprotective effect against ischemia reperfusion brain injury, the mechanism of protection may be related to inhibiting the injury of free radicals.
出处
《中国医药指南》
2010年第5期27-29,共3页
Guide of China Medicine
关键词
脑缺血/再灌注
丙二醛
超氧化物歧化酶
瑞芬太尼
Cerebral ischemia-reperfusion
Malondialdehyde
Super oxide dismutase
Remifentanil
