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用复性电泳技术研究金线鱼消化道蛋白酶的分布 被引量:1

Activity distribution of digestive proteases from Nemipterus Virgatus by non-denatured electrophoresis
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摘要 采用复性电泳和活力检测方法对3组不同体重的金线鱼(Nemipterus virgatus)消化道蛋白酶的分布的研究表明:金线鱼各消化器官蛋白酶活性均随实验鱼体重增加而上升,但与体重增加不成比例。不同体重的金线鱼的消化道蛋白酶不仅存在活力差异,在种类和数量上也存在明显差别。3组鱼的消化道蛋白酶单位酶活力从高到低依次为幽门盲囊>胃>前肠>中肠>后肠>肝胰脏,但以总活力计算时,仍以胃部蛋白酶的总活力最大。 The distribution and properties of three groups of Nemipterus virgatus were studied by non-denatured electrophoresis.The results showed that the activities of protease in all digestive organs went up with body weight increasing, but there was not ratio between the activity and body weight.Not only the activity but also the species and amounts were different with the changing of body weight.The protease activity was in descending order, pylorus caeca 〉 stomach 〉 foregut 〉 midgut 〉 hindgut 〉 hepatopancreas. But maximal total protelytic activity was found in stomach.
作者 朱美娟 陶红
出处 《食品工业科技》 CAS CSCD 北大核心 2010年第2期114-117,121,共5页 Science and Technology of Food Industry
关键词 金线鱼 复性电泳 消化道蛋白酶 Nemipterus virgatus(Houttuyn) non-denatured electrophoresis digestive proteases
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