摘要
鸡血浆样品用甲醇沉淀蛋白后,过DikmacC18柱(100mm×4.6mm,5μm),柱温:室温;流动相:甲醇-0.02mol/L乙酸胺缓冲液(28∶72),pH4.9;流速:1.0mL/min;检测波长:254nm;检测器:紫外检测器;进样量:10μL。结果表明,检测线性范围为0.5～32.0μg/mL(r=0.9999);最低检测浓度为0.25μg/mL;回收率分别为92.5%、96.4%、98.9%;日内和日间变异系数均小于5%。该方法操作简便、灵敏、重现性好,适用于头孢拉定的临床药代动力学研究。
After the plasma protein was precipitated, Cefradine was separated well on a Dikmac C,s clumn （100 mm×4.6 mm ,5μm）under room temperature. The mobile phase was a mixture of methanol-0.02mol/L ammonium acetate buffer solution （28：72 ,pH 4.9）at a rate of 1.0 mL/min. The UV detection was set at 254 nm. The results showed that the calibration curve Was linear in the range of 0.5-32.0 μg/mL （r=0.9999）. The lowest limit was 0.25μg/mL and the recoveryies were 92.5% ,96.4%, 98.9%. The RSD values of whithin-day and between-day precision were less than 5%. This method is simple, sensitive, accurate and reproducile. It is applicable for pharmacokinetic study of Cefraddine.
出处
《广东农业科学》
CAS
CSCD
北大核心
2010年第2期146-148,共3页
Guangdong Agricultural Sciences
