摘要
目的观察苯扎贝特对血小板源生长因子诱导的大鼠血管平滑肌细胞增殖及小凹蛋白1表达的影响并探讨其机制。方法体外植块贴壁法培养大鼠胸主动脉血管平滑肌细胞,噻唑兰比色法检测各组细胞增殖情况,W estern B lotting法观察各组小凹蛋白1的表达情况。结果不同浓度的苯扎贝特对血小板源生长因子诱导的血管平滑肌细胞增殖均有一定的抑制作用,随浓度增加,其抑制作用增强(P<0.01)。血小板源生长因子干预组小凹蛋白1的表达较对照组明显降低(P<0.01),而用不同浓度的苯扎贝特干预后小凹蛋白1的表达明显升高(P<0.01),且随着苯扎贝特浓度增加,小凹蛋白1的表达明显增加。结论苯扎贝特抑制血小板源生长因子诱导的血管平滑肌细胞增殖的同时可以上调小凹蛋白1的表达。
Aim To observe the effects of bezafibrate on the expression of caveolin-1 and the proliferation of vascular smooth muscle cells（VSMC） induced by platelet-derived growth factor（PDGF） and investigate the mechanism of it.Methods VSMC were cultured in vitro,the proliferation of VSMC were obtained through thiazolyl blue colorimetric technique（MTT）.The expression of caveolin-1 was assayed by Western Blotting.Results Compared with the control group,PDGF could increasingly induce the proliferation of VSMC（P0.01）.Bezafibrate inhibited the proliferation of VSMC induced by PDGF in concentration-dependent manner（P0.01）.Compared with the control group,PDGF could decrease caveolin-1 protein expression（P0.01）.Bezafibrate increase the expression of caveolin-1 compared with the PDGF group（P0.01）.With the increase of Bezafibrate＇s concentration,the expression of caveolin-1 could increase accordingly.Conclusion Bezafibrate can decrease the proliferation of VSMC induced by PDGF and increase the expression of caveolin-1 protein.
出处
《中国动脉硬化杂志》
CAS
CSCD
北大核心
2009年第12期1002-1004,1032,共4页
Chinese Journal of Arteriosclerosis
关键词
苯扎贝特
血管平滑肌细胞
细胞增殖
血小板源生长因子
小凹蛋白1
细胞培养
Pathophysiology
Bezafibrate
Vascular Smooth Muscle Cells
Proliferation
Platelet-Derived Growth Factor
Caveolin-1
Cell Culture
