摘要
目的:建立浅表性膀胱癌模型并以活体荧光成像系统观察肿瘤生长情况。方法:利用脂质体将增强型绿色荧光蛋白(EGFP)表达质粒转染人膀胱癌细胞株KU-7,G418筛选稳定表达EGFP的克隆。采用经尿道膀胱灌注法使肿瘤细胞种植于膀胱黏膜。活体荧光荧光成像系统直接观察肿瘤细胞生长及形成瘤体的过程。结果:建立了转染率接近100%的人膀胱癌KU-7/EGFP细胞系,在体外及裸小鼠体内均能够长期稳定表达绿色荧光蛋白。活体荧光成像观察发现,1～4周随着肿瘤体积逐渐增大,肿瘤的发光随着观察时间的延长而增加。结论:利用绿色荧光蛋白标记膀胱肿瘤细胞,建立一种浅表性膀胱癌模型,为连续动态实时观察和准确评价自然状态下肿瘤细胞生长过程提供了新的手段。
OBJECTIVE：To establish a mouse model of superficial bladder cancer and to detect the growth of bladder cancer cell with whole-body fluorescent imaging system.METHODS：Human bladder cancer cells were transfected with enhanced green fluorescent protein （EGFP） gene by Lipofectamine2000 and screened with G418 to produce the stable EGFP-expressing clone. Then the labeled bladder cancer cells were implanted into the nude mice bladder by intravesical instillation. The dynamic growth of xenografted tumor was observed by whole-body fluorescent imaging system for subsequent 4 weeks.RESULTS：We successfully built up a human bladder cancer KU-7/EGFP cell line with transfection rate of nearly 100%,which can stably express high level of EGFP protein in vitro and in vivo. In vivo fluorescence miaging found that the mean fluorescent intensity gradually increased with the increase in tumor volume in nude mice from one week to four weeks.CONCLUSION：This animal model can play a important role in the study of the mechanism of growth and drug discovery in superficial bladder cancer.
出处
《中华肿瘤防治杂志》
CAS
2009年第24期1932-1934,共3页
Chinese Journal of Cancer Prevention and Treatment
基金
北京市自然科学基金(7052025)
关键词
膀胱肿瘤
绿色荧光蛋白
模型
bladder neoplasms
gree fluorescent protein
model
