摘要
用体外药物连续选择法建立了一株人卵巢癌多药耐药细胞系。发现耐药细胞内谷胱甘肽含量、谷胱甘肽过氧化物酶活性、谷胱甘肽转硫酶活性均增加，从而使细胞内药物失活增加；细胞内药物作用靶减少，拓扑异构酶活性降低；细胞内药物积聚量减少，但未见P-糖蛋白过度表达。提示卵巢癌耐药性的产生与药物失活增加、药物作用靶减少及细胞内药物积聚量减少有关。
in order to study the multidrug resistant phenomena, we established a human ovariancarcinoma MDR cell line COC1/DDP in vitro by progressive drug selection. We analyzed the drug resistant mechanisms and detected that the amount ofglutathione, the activity of glutathione peroxidaseand glutathione -S-transferase in COC1 /DDP cellswere all obviously higher than those of the parentalcell COC1, which resulted in more drug inactivation. The activity of DNA topoisomerase was reduced. The cellular drug accumulation was reducedand there was no P-glycoprotein expressed in COC1/DDP cells. These results indicate that MDR phenomena are related to the addition of drug inactivation, the reduction of drug,s target and cellular drugaccumulation.
出处
《西安医科大学学报》
CSCD
1998年第3期411-414,共4页
Journal of Xi'an Medical University(Chinese)
关键词
卵巢癌细胞系
多药耐药性
耐药机制
human ovarian carcinoma cell line
multidrug resistance
drug inactivation
DNA topoisomerase
P-glycoprotein
