摘要
目的分析云南省西双版纳地区基诺族、傣族和傻尼族人群CⅡTAG-944C多态性的分布，并探讨其多态性与HBV感染的相关性。方法运用PCRN序的方法对三民族人群CⅡTAG-944C位点进行基因分型，并分析其基因多态性与HBV感染的相关性。结果（1）馒尼族人群HBV感染率及HBsAg携带率显著高于基诺族和傣族，差异有统计学意义（馒尼族抛基诺族χ^2值分别为135．196和10．361，P值分别为0．000和0．001；馒尼族vs．傣族χ^2值分别为96．783和8．748，P值分别为0．000和0．003）。（2）馒尼族人群CIITAG-944C位点基因型与等位基因分布频率与其他两民族显著不同，CC基因型与C等位基因频率升高，而GG基因型与G等位基因频率则降低，差异有统计学意义（馒尼族椰．基诺族χ^2值分别为11．841和12．208，P值分别为O．003和0．000；馒尼族vs.傣族χ^2值分别为23．902和20．220，P值均为0．000）。（3）CIITAG-944C基因型和等位基因分布频率在基诺族人群HBV感染组与对照组中差异有统计学意义（f值分别为6．150和4．911，P值为0．046和0．027）；而HBsAg^＋组与HBsAg^-组相比，在馒尼族人群和三民族总体人群中分布均有差异（傻尼族χ^2值分别为8．650和5．034，P值分别为0．013和0．025；总体人群χ^2值分别为13．047和9．416，P值分别为00．001和0．002）；HBsAg^＋组CC基因型和C等位基因分布频率显著升高。（4）以非条件logistic回归模型校正混杂因素后，在C隐性模式下（CC／GG＋GC），HBsAg^＋组与HBsAg^-组差异有统计学意义（P=O．000；OR=2．964；95％CI：1．609～5．460）。结论CIITAG-944C基因型和等位基因频率具有民族差异，并且其多态性与HBsAg携带密切相关，基因型为CC纯合子的HBV感染患者更易发展成为HBsAg携带者。
Objective To explore the relationship between I-IBV infection and the genotypes and allele frequencies of C II TA G-944C gene polymorphism in three minority populations （Jinuo, Dai and Aini population） in Xishuangbanna district, Yunnan province. Methods Polymerase chain reaction and sequencing method were used to study the genotypes and allele frequencies distributions of C II TA G-944C gene polymorphism in those three populations. Relationship between the genotypes distribution and HBV infection results were also analyzed. Results The rates on HBV infection and HBsAg carrier status in Aini minority population were 89.2% and 16.3%, which were significantly higher than in Jinuo （27.9% and 3,9%, χ^2= 135.196 and 10.361, P=0.000 and 0.001） and Dai population （44.9% and 6.6%,χ^2= 96.783 and 8.748 ,P=0.000 and 0.003 ） while among Aini population it was significantly different with the other two minority populations. The CC genotype and C allele frequencies were more distributed in Aini population than in the other two minority populations. In contrast, the GG genotype and G allele frequencies were lower than the other two minority populations, with 22 rates between Aini and Jinuo population were 11.841 and 12.208 and the P as 0.003 and 0.000 respectively while the 22 rates between Aini and Dai population were 23.902 and 20.220 with P value as 0.000 and 0.000. The genotypes frequencies of C 11 TA G-944C was significantly different in the infected individuals （IF） group and health control （HC） group in Jinuo population （χ^2=6.150 and 4.911, P=0.046 and 0.027）. When compared with HBsAg＋ group and HBsAg- group, the genotypes and allele frequencies were different in Aini population and the total three minority populations （ 22 rates in Jinuo minority were 8.650 and 5.034 with P values as 0.013 and 0.025）. However, the 22 rates in the whole population were 13.047 and 9.416 with P values as 0.001 and 0.002, respectively. The distribution of CC genotype and C allele gene in HBsAg^＋ group was increasing. Data from non-condition logistic regression analysis and adjusting for confounding factors, the HBsAg＋ group had a significantly increase of HBsAg group under the C allele Recessive Model （P=0.000; 0R=2.964; 95% CI： 1.609-5.460）. Conelusion The genotypes and allele frequencies distribution of C II TA G-944C were different in the three ethnic populations. Polvmorohism of this gene was closely associated with HBsAg carrier. The CC genotvpe patients were more easily to become HBsAg carrier.
出处
《中华流行病学杂志》
CAS
CSCD
北大核心
2010年第2期146-150,共5页
Chinese Journal of Epidemiology
基金
基金项目：云南省自然科学基金（20030017Z）
云南省科技厅-昆明医学院应用基础联合专项资金（2008CD044）
