摘要
首次应用高效液相色谱-二极管阵列检测器-质谱联用技术(HPLC-DAD-MS)鉴定出紫皮豇豆主要含有三种花色苷,分别为飞燕草色素、矢车菊色素、天竺葵色素的葡萄糖苷,其中矢车菊葡萄糖苷相对百分含量达到93.4%;应用pH示差法测定出其单体花色苷的含量为57.15mg/100g鲜重;应用DPPH法测定结果显示,花色苷粗提液有较强的抗氧化能力。
Purple cowpeas( Vigna unguiculata L.)were extracted with of 0.1 % HCI in methanol solution, purified on a C-18 solid cartridge, quantified by the pH differential method, and characterized by means of HPLC- DAD- IT/ TOFMS analysis for the first time.Antioxidant activity of extracts was screened using DPPH radical scavenging methods.The content of anthocyanins in the purple cowpeas was 57.15mg/100g F-W.Three kinds of anthocyanin riched in the extracts which exhibited strong antioxidant activity were identified, they were delphinidin 3-O-glucoside,cyanidin3-O-glucoside, pelargornidin 3-O-glucoside.
出处
《食品工业科技》
CAS
CSCD
北大核心
2010年第3期155-157,161,共4页
Science and Technology of Food Industry
关键词
紫皮豇豆
花色苷
抗氧化性
purple cowpea
anthocyanins
antioxidant activity