摘要
采用正交实验设计,分别对超声萃取法(UE)和超声-索氏萃取法(USE)测定土壤中总石油烃(TPH)的影响因素进行分析,获得优化条件。采用以上两种方法及传统的索氏萃取法(SE)对新鲜石油污染土、微生物修复土和老化植物修复土进行提取。结果表明:超声萃取法的优化条件为选用15mL三氯甲烷,萃取4次;超声-索氏萃取法的优化条件为60W超声萃取10min,萃取剂用量125mL,萃取6h;超声-索氏萃取法平均回收率最高,为102.98%,且数据稳定可靠;其次是超声萃取法,为99.69%;而传统的索氏萃取法最低,为97.31%。研究证明超声-索氏分析法在缩短测定时间的同时提高了回收率,可作为不同土壤中总石油烃(TPH)含量测定的新方法。
The influencing factors of ultrasonic extraction（ UE） and ultrasonic-soxhlet extraction（ USE） were analyzed based on orthogonal experimental design. The optimum analytical conditions of two methods were determined. The three different methods： UE,USE and traditional soxhlet extraction（ SE） were compared by simultaneous analysis with three different soil samples： fresh contaminated soil,soil after microbial remediation and soil after phytoremediation. The optimum condition of the UE was extracting 4 times with chloroform, 15 mL extractant every time. The optimum condition of the USE method was ultrasonic for 10 min at 60 W, followed by soxhlet extraction with 125 mL extractant for 6 h. Compared the three different methods,USE method showed the best result with the highest average extraction rate of 102. 98% ,the UE method with average extraction rate of 99. 69% ,and the SE method with low average extraction rate of 97. 31% . The research indicates that USE method is effective in determining soil total petroleum hydrocarbons（ TPH） within shorter time and higher efficiency and can be used as a method for determining TPH content in different soils.
出处
《分析化学》
SCIE
EI
CAS
CSCD
北大核心
2010年第3期417-420,共4页
Chinese Journal of Analytical Chemistry
基金
863重点项目"微生物-植物联合原位生态修复技术处理中低浓度石油污染土壤"(No.2007AA061201)
天津市应用基础及前沿技术研究计划(No.09JCYBJC08800)
中国科学院知识创新工程(No.kzcx1-yw-06-03)资助项目
关键词
土壤
总石油烃
超声-索氏萃取法
正交实验设计
Soil
Total petroleum hydrocarbons
Ultrasonic-soxhlet extraction
Orthogonal experimental design
