摘要
通过对GenBank发表的ALV的基因序列的分析,针对J型ALV保守区设计并合成1对特异性引物。通过对反应条件的优化,确定了检测ALV的PCR检测方法,对来源于江苏地区不同草鸡养殖基地的10份病料组织样本提取DNA进行PCR扩增。结果对疑似J亚群ALV病料和正常鸡肝脏组织的病原核酸检出率分别为90%和0,同时将扩增的目的基因进行克隆测序,并与GenBank中的参考毒株进行比较,结果表明目的基因片段序列长300 bp左右,与参考毒株核苷酸序列同源性为97%以上。试验表明,江苏地区草鸡群中确实存在J亚群鸡白血病病毒感染的情况。
According to the conserved gene sequences of ALV in GenBank, a pair of the specific primers were designed. Through the optimization of reaction conditions, the PCR detection method was established. Then the ten tissue samples separated from different local chicken breeding bases in Jiangsu province were detected by PCR. The results showed that 90% of the suspected samples were ALV positive and the normal chicken liver tissue was ALV negative. The amplified target gene was cloned, sequenced and compared with the reference nucleotide sequence in GenBank, the results showed that the target gene fragment was 300 bp, and the homology of nucleotides was more than 97%. The study showed that J - subtype ALV existed in the local chickens in Jiangsu province.
出处
《江西农业学报》
CAS
2010年第3期5-7,共3页
Acta Agriculturae Jiangxi
基金
江苏省农业自主创新资金项目[cx(08)142]
