摘要
提供北京汉族群体9个STR基因座的频率分布资料，了解其在法医学中的应用价值。应用PCR技术对9个STR基因座分3组进行复合扩增，经PAG电泳分离、银染，扫描仪扫描，计算机判读并保存结果，对北京地区汉族无关个体9个基因座的基因频率分布进行调查。结果显示，上述9个基因座的杂合度为0．6419～0．8092，多态性信息总量为0．9999，鉴别机率为0．9999，匹配机率为2．0×10-9和非父排除率为0．9985。STR3组9个基因座的综合检验可应用于法医学个体识别和亲子鉴定，并达到同一认定的标准。
A PCR multiplexing method of nine STR loci was established. Nine loci were divided into three groups which were group Ⅰ (CSF1PO, TPOX, TH01 ), group Ⅱ (F13A01, FESFPS, vWA) and group Ⅲ (D16S539, D7S820, D13S317 ). The multiplexing method was carried out consequently as PCR, PAGE, silver staining, scanning,reading and saving the results in the computer. The allele frequency distribution of nine STR loci of 195～236 unrelated individuals of Han populations in Beijing has been studied. The hetrozygosity (H), discriminating power (Dp), probability of exclusion (PE), polymorphism information content (PIC) and the probability of matching were 0. 6419 ~ 0. 8092,0. 9999, 0. 9985, 0. 9999 and 2.0 ×10 -9 respectively. It is suggested that this multiplexing of nine STR loci can be used in personal identification and paternity testing.
出处
《中国法医学杂志》
CSCD
1998年第4期210-212,共3页
Chinese Journal of Forensic Medicine
关键词
短串联重复序列
PCR
个体识别
亲子鉴定
Short tandem repeat (STR) Polymerase chain reaction (PCR) Multiplexing Patemity testing Personal identification
