摘要
为了降低aFGF的生产成本,结合植物生物反应器的优点,就aFGF在转基因烟草中的表达进行了探索。将aFGF基因插入植物表达载体pBI121中,获得了含有aFGF基因的植物表达载体pBI121-TΩAB-aF,利用农杆菌介导法将基因转化烟草,转基因烟草在含有卡那霉素和头孢霉素的培养基培养,获得具有卡那抗性的转基因烟草植株,通过PCR检测、RT-PCR检测及Western blot检测证实外源基因已经在烟草中成功表达。为植物生物反应器生产aFGF提供了理论基础。
Acid fibroblast growth factor（aFGF） has great potential in clinical application,but it is very expensive.In order to reduce the cost of production and to make full use of the merits integrated with plant bioreator,we have explored the aFGF in transgenic Tobacco expression.AFGF gene was inserted into plant expression vector pBI121;the acquired plants contained aFGF gene expression vector pBI121-TOAB-aF.Using Agrobacterium-mediated gene transformation of Tobacco and using transgenic Tobacco containing kanamycin and cephalosporin culture medium,we obtained kanamycin resistant transgenic Tobacco plants.PCR detection,RT-PCR detection and Western blot detection confirmed that foreign genes were successfully expressed in Tobacco.These data could serve as a theoretical foundation on which to use the plant bioreactor for production of aFGF.
出处
《生物医学工程学杂志》
EI
CAS
CSCD
北大核心
2010年第1期126-131,共6页
Journal of Biomedical Engineering
基金
十一五"863"计划生物反应器重大专项资助项目(2007AA100503)
吉林省科技发展重点计划资助项目(20070922)
高等学校科技创新工程重大项目培育基金资助项目(70S018)
长春市科技计划资助项目(06GG150)
