摘要
在抗寒锻炼前,对当年生大叶黄杨（Euonymus japonicus）扦插苗喷施不同浓度水杨酸,用电阻抗图谱（EIS）法和电导（EL）法估测茎的抗寒性,以探明抗寒锻炼期间水杨酸对大叶黄杨抗寒性和电阻抗图谱参数的影响,找到适合不经冷冻处理估测大叶黄杨茎抗寒性和经冷冻处理后测定其抗寒性的EIS参数。结果表明,水杨酸处理能够提高大叶黄杨茎的抗寒性,最适浓度为5.0mmol·L^-1;不经冷冻处理茎的EIS参数电阻率r和r1、胞外电阻率re、胞内电阻率ri、弛豫时间τ、弛豫时间分布系数ψ与EL法测定的抗寒性有较高的相关性（r=0.70-0.87）,说明不经冷冻处理样本用以上参数估测大叶黄杨茎抗寒性是可行的,r1为最佳参数;冷冻处理后茎的re、τ、ri求得的抗寒性与EL法测定的抗寒性有较高的相关性（r=0.85-0.94）,说明冷冻处理后re、τ、ri可以作为测定大叶黄杨茎抗寒性的参数,re为最佳参数。
Current-year cutting plants of Euonymus japonicus were treated with different concentration salicylic acid（SA）before frost hardening,and their frost hardiness during the period of the hardening was assessed by electrical impedance spectroscopy（EIS）and electrolyte leakage（EL）,aimed to explore the effects of SA on the frost hardiness and the EIS parameters of E.japonicus,and to search for the suitable EIS parameters to evaluate the frost hardiness of E.japonicus stems with and without artificial freezing.Treating with SA increased the frost hardiness of E.japonicus stems,and the optimal concentration of SA was 5.0 mmol·L^-1.For the non-frost-exposed stems,the resistances r and r1,extracellular resistance re,intracellular resistance ri,relaxation time τ,and distribution coefficient of relation time ψ had high correlations with the frost hardiness measured by EL（r=0.70-0.87）,suggesting that these EIS parameters could be used to measure the frost hardiness of E.japonicus without artificial freezing,and r1 was the best parameter.For the frost-exposed stems,the re,τ,and ri were highly correlated with the frost hardiness（r=0.85-0.94）,indicating that re,τ,and ri could be the suitable parameters to measure the frost hardiness of E.japonicus after artificial freezing,and re was the best parameter.
出处
《生态学杂志》
CAS
CSCD
北大核心
2010年第3期460-466,共7页
Chinese Journal of Ecology
基金
河北农业大学留学回国人员启动基金资助项目(200406)
关键词
大叶黄杨
抗寒锻炼
抗寒性
水杨酸
电阻抗图谱
Euonymus japonicus
frost hardening
frost hardiness
salicylic acid
electrical impedance spectroscopy
