摘要
目的：研究白细胞（WBC）参与缺血再准流肾损伤的作用高峰时间。方法：将24只家兔随机分为对照、缺血再灌流2小时（IR－2小时）和缺血再灌流24小时（IR－24小时）三组，分别观察血中白细胞（WhC）和过氧化脂质（LPO）含量、左肾系数（LRC）和肾超微结构的改变。结呆：IR－2小时组同对照组比较，WBC和LPO含量明显升高，LRC明显增大（P＜0．05），且WBC与LPO、LRC之间均呈显著正相关（P＜0．05）。IR-24小时组在24小时时，WBC与LPO之间仍呈显著正相关（P＜0．05），但WBC与LRC之间无相关关系，同IR-2小时时比较，WBC和LPO含量明显下降（P＜0．05）；同对照组比较，LRC明显增大（P＜0．05）。IR-2小时和IR-24小时组肾组织超微结构改变一致，肾小球和肾小管均发生明显的变性、坏死。结论：白细胞参与肾损伤，其作用的高峰期在再灌流2小时左右。
To study the effect-time relationship of WBC in renal acute ischemia- eperfu-tion injury. Methods: 24 rabbits were randomly divided into three groups: the control (n=8) 2h ofreperfusion(IR-2h, n = 6) and 24h of repeffosion(IR-24h, n =10)after 1h of renal ischemia. The bloodWBC count and lipid peroxide(LPO) control, changes in left renal coefficient and the ultrastructure ofrenal tissue were observed. Results: WBC and LPO puls LRC increased markedly in IR-2h group com-pared with that of control group(P<0.05). There was a positive correlation between WBC and LRC be-sides WBC and LPO in IR-2h group(P < 0.05); A positive correlation was also observed betweenWBC and LPO(P < 0. 05), however, no correlation between WBC and LRC at 24th hour in IR-24hgroup. The count of WBC and content of LPO at 24th hour of IR- 24h group decreased significantly, butthe LRC at 24th hour of IR- 24h group increased considerably compared with that of control group. Theglomerular and tubular degeneration and necrosis occurred in IR-2h and IR- 24h groups. Conclusion:The results suggest that there is a time- effect relationship of WBC in renal injury, and the maximal effectexsists at about 2h after ischemia-reperfusion.
出处
《泸州医学院学报》
1998年第5期373-375,共3页
Journal of Luzhou Medical College
基金
四川省卫生厅科研基金
关键词
白细胞
缺血再灌流
肾损伤
WBC
Acute ischemia reperfusion
Renal injury
