摘要
以玉米为材料,采用序列分析及半定量RT-PCR等方法对ZmCIPK基因功能进行了初步研究.序列分析结果推测,AY104819,EU974290和EU968441均属于CIPK蛋白家族.半定量RT-PCR结果表明,NaCl和ABA均能迅速诱导玉米基因AY104819,EU974290和EU968441的表达.
Sequence analysis and semi-quantitative RT-PCR were used to study the preliminary function of the CBL-interacting protein kinase from maize(ZmCIPK).From the result of sequence analysis,it is speculated that,AY104819,EU974290 and EU968441 all belong to CIPK protein family.Semi-quantitative RT-PCR analysis indicated that,maize gene AY104819,EU974290 and EU968441 can be rapidly induced by NaCl and ABA.
出处
《河南农业大学学报》
CAS
CSCD
北大核心
2010年第1期12-18,27,共8页
Journal of Henan Agricultural University
基金
河南农业大学引进人才科研启动基金项目(30500247)