摘要
根据克隆的花生油酸脱氢酶基因序列,选取高度保守的260 bp片段,插入植物双元表达载体pFGC1008中,构建完成具有反向重复序列的pFGC/2nd表达载体,利用RNAi以抑制内源目的基因表达,为获得高油酸亚油酸比值的花生种质奠定基础.
According to the sequence of oleate desaturase gene from peanut,the highly conservative sequence 260 bp was selected and cloned into the expression vector,pFGC1008,to generate reverse repeat of the anti-expression vector pFGC/2nd.The RNAi technique was used to inhibit the expression of oleate desaturase gene in peanut,in order to obtain transgenic lines with high oleic and linoleic acid ratio.
出处
《河南农业大学学报》
CAS
CSCD
北大核心
2010年第1期24-27,共4页
Journal of Henan Agricultural University
基金
国家自然科学基金项目(30971800)
河南省重大科技攻关项目(092101110500)
