摘要
以40株鸡致病性大肠杆菌为作用对象分析噬菌体Bp7的裂解谱,并用透射电镜较系统地观察分析噬菌体Bp7侵染并裂解大肠杆菌的过程。结果显示,噬菌体Bp7可裂解40株鸡致病性大肠杆菌中的14株,裂解率达35%。噬菌体Bp7头部外廓呈六边形,平均直径约93nm,尾长约106nm,有尾鞘。噬菌体Bp7侵染大肠杆菌后30min时处于隐蔽期,45min时处于成熟阶段,60min时已经进入释放期,一个溶菌周期大约为60min。试验为噬菌体作为抗生素替代品治疗细菌病的研究提供了理论基础。
The aim is to observe and analyze the infection process of E.coli by bacteriophage Bp7 systematically by TEM and its lysis spectrum. The results show that, Bp7 can lyse 14 in 40 strains of E.coli and its lysis rate is 35%. Bp7 has a hexagonal head with diameter of 93nm and connected with a flexible tail which mean length is 106 nm. The tail has tail sheath. E.coli via its tail wire. In the first 30 min, the infection is in the hidden phase, and maturity phase appears at 45mpi （minute post infection）, release period is at 60mpi, and a lytic cycle is about 60 min. This study provides a theoretical basis for bacteriophage as an alternative to antibiotics in the treatment of bacterial diseases.
出处
《中国农学通报》
CSCD
北大核心
2010年第9期18-21,共4页
Chinese Agricultural Science Bulletin
基金
山东省自然科学基金"大肠杆菌多价噬菌体尾丝蛋白结构与功能研究"(ZR2009DM009)
关键词
大肠杆菌
噬菌体
侵染过程
裂解谱
Eseheriehia coli
bacteriophage
infection process
lysis spectrum
