摘要
目的:研究异丙肾上腺素对失神经骨骼肌萎缩中NF-κB、MuRF1表达变化的影响,探讨其防治失神经骨骼肌萎缩的机制。方法:选用wista大鼠54只,制作成标准右侧坐骨神经离断、腓肠肌失神经支配模型,随机分为对照组和药物组,应用RT-PCR和Western Bloting检测不同时段的NF-κB、MuRF1mRNA和蛋白质表达水平,联合肌湿重分析其相关性。结果:失神经支配后腓肠肌NF-κB、MuRF1mRNA和蛋白质表达持续增加(P<0.05)。药物组肌湿重和肌细胞横截面积比对照组同时间点明显增加(P<0.05),NF-κB、MuRF1mRNA和蛋白质表达则明显降低(P<0.05)。结论:异丙肾上腺素可以通过抑制NF-κB/MuRF1途径有效防治失神经骨骼肌萎缩。
Objective： To investigate the change of NF - κB and muscle Ring Finger - 1 （ MuRF1 ） on denervation muscle atrophy intervated with isoprel. Methods：Levels of NF - κB and MuRF1 mRNA and protein in gastrocnemins muscle at different time after denervation were detected by RT - PCR and western Blotting. The ratio of muscle wet weight was analyzed for comparison. Result： The level of mRNA and protein of NF - κB and MuRF1 increased correpondingly at different time after denervation （ P 〈 0.05 ） but The group with isoprel decreased at the same time. Conclusion：the change of mRNA and protein presented consistency at different time, and MuRF1 is actively correlated to with increase of levels of NF- κB, indicating that NF - κB/MuRF1 pathway plays an importance role in denervation atrophy of the gastrocnemius,and isoprel is possible to delay denervation muscle atrophy by inhibite the pathway of NF - κB/MuRF1.
