摘要
建立一种基于表面等离子体共振技术(SPR)测定猪肉中磺胺类药物残留的新方法。采用传感芯片为共振芯片,以0.1mol/LNaOH溶液为再生溶液,HBS-EP为缓冲溶液,流速为80μL/min,运用SPR技术测定猪肉中磺胺类药物残留总量。结果表明,磺胺类药物在0.5～50ng/mL范围内,传感芯片表面所产生的相对共振强度与质量浓度有良好的响应关系,平均回收率为75.7%～99.2%,精密度实验RSD为1.3%(n=6),检测限为2.5μg/kg。该方法简便、灵敏,可以为产品的安全与质量控制提供快速分析方法。
A new method based on surface plasmon resonance（SPR） was established to determine total sulfonamide residue in pork.A commercial sensor chip CM5 was activated and derivatized with HBS-EP buffer at a flow rate of 80 μL/min,blocked with 1 mol/L cholamine aqueous solution at a flow rate of 10 μL/min,and regenerated with 0.1 mol/L sodium hydroxide aqueous solution to obtain a resonance chip.Relative resonance intensity generated from resonance chip surface presented a good response to sulfonamide concentration ranging from 0.5 to 50 ng/mL.The method exhibited an average spike recovery range of 75.7% to 99.2% at three levels and a limit of detection of 2.5 μg/kg.Reproducibility experiments gave a RSD of 1.3%（n = 6）.This method presents the benefits of simplicity and high sensitivity,thereby providing a rapid analytical approach for the control of quality and safety of meat products.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2010年第6期216-219,共4页
Food Science
