摘要
目的:初步调研市售纯牛奶雌二醇(E2)和孕酮(P4)含量现状。方法:收集同季节6个品牌(A、B、C、D、E和F品牌,各品牌随机采集6个批号)共36个批号市售纯牛奶,采用免疫化学发光分析法检测经硫酸酯酶水解的乳清总E2(TE2)和P4。结果:6个品牌乳清TE2差异无统计学意义;F品牌乳清P4高于C品牌(P<0.05),其余各品牌间差异均无统计学意义。36个批号中,乳清TE2和P4最大值与最小值之比分别为2.32和6.67。A、B、C、D、E和F品牌批号间乳清TE2的最大值与最小值之比分别为1.38、1.60、1.82、1.79、2.17和1.68,其P4的最大值与最小值之比分别为3.19、5.98、2.89、1.38、1.86和1.67。6个品牌的乳清TE2和P4均值的最大值与最小值之比分别为1.25和3.05。结论:市售纯牛奶品牌间乳清P4存在差异,且同品牌不同批号波动较大;批号间乳清TE2也有一定差异。
Six commercial brands（A,B,C,D,E and F） of whole milk produced in Wuxi city or other regions from February to April were sampled two batches every month to obtain 36 samples.A survey of estradiol（E2） and progesterone（P4） contents in the whole milk samples measured by immuunochemiluminpmetric assay was carried out in this study.The content of progesterone present in whey obtained from whole milk by centrifugation was directly measured.Before estradiol measurement,whole milk was required to be hydrolyzed with sulfatase.Results showed that no statistic difference in TE2 content（a quarter of E2 content） was observed among 6 commercial brands of whole milk surveyed in this study.Brand F exhibited a higher P4 content than brand C（P0.05）,while other brands do not differ statistically in P4 content.Among 36 whole milk samples,the maximum value of TE2 content was 2.32 higher than the minimum value and the ratio of maximum to minimum P4 contents was 6.67.For brands A,B,C,D,E and F,the ratio of maximum to minimum TE2 contents were 1.38,1.60,1.82,1.79,2.17 and 1.68,and the ratio of maximum to minimum P4 contents 3.19,5.98,2.89,1.38,1.86 and 1.67,respectively.The ratios of maximum average to minimum average values of TE2 and P4 contents among 6 brands were 1.25 and 3.05,respectively.The above results indicate that 6 commercial brands of whole milk significantly differ in P4 content,and there is a wide fluctuation range of P4 content in different bitches of the same brand.Moreover,some difference in TE2 content is also found in different bitches of the same brand.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2010年第6期252-254,共3页
Food Science
基金
第五批(2007年度)苏州大学医学发展基金项目(21)
