摘要
依据鸡A-FABP和H-FABP基因及参照基因GAPDH序列设计引物,以鸡心肌、胸肌和腿肌、肝脏和腹脂总RNA逆转录合成的cDNA为模板,GAPDH基因为参照基因,应用SYBRGreenI实时荧光定量PCR检测如皋黄鸡和安卡红鸡A-FABP和H-FABP基因的差异表达。结果表明:A-FABP、H-FABP基因和参照基因GAPDH基因融解曲线为单峰,无杂峰及二聚体,其扩增效率分别为99.7%、99.8%和100.0%。如皋黄鸡H-FABP基因在心肌、胸肌和腿肌的差异表达量分别是安卡红鸡的0.0860、0.0680倍和0.0580倍(P<0.05)。H-FABP基因mRNA表达量与肌内脂肪含量呈显著负相关。如皋黄鸡A-FABP基因在腹脂和肝脏的表达量分别是安卡红鸡的15.9640倍和10.9640倍(P<0.05),而在心肌、胸肌和腿肌的表达量差异不显著。
The relative expression in heart and adipocyte fatty acid-binding protein （H-FABP and A-FABP ） genes was detected using real time quantitative RT-PCR in Rugao and Anka chicken. The primers were designed according to the sequences of H-FABP, A-FABP and GAPDH gene. GAPDH gene was the internal reference gene. The cDNA fragment was amplified from leg muscle, breast muscle, cardiac muscle, liver and abdominal fat mRNA; H-FABP, A-FABP and GA PDH gene expression was detected using SYBR Green I real-time fluorescent quantity PCR. Melting curve analysis showed a single peak of H-FA BP, A-FABP gene and GA PDH gene. PCR efficiency of H-FA BP, A-FABP and GA PDH was 99.7%, 99.8% and 100.0% respectively. The relative H-FABP mRNA level （ratio of Anka to Rugao） in cardiac muscle was 0.0860, 0.0680 and 0.0580, significantly higher than that of the other tissues （P 〈 0.01）. H-FABP mRNA expression level was significantly negative correlation with IMF contents. The relative A -FABP mRNA level （ratio of Anka toRugao） in abdomen fat and liver was 15.9640 and 10.9640, significantly higher than that of the other tissues （P 〈 0.01）. The relative A -FABP mRNA level in abdomen fat was higher than that of Liver. There were no significant differences between leg muscle, breast muscle and cardiac muscle of relative A -FA BP mRNA levels.
出处
《中国畜牧杂志》
CAS
北大核心
2010年第7期1-4,共4页
Chinese Journal of Animal Science
基金
国家科技支撑计划(2008BADC1B03
2008BADC1B01
2006BDA01A09)
国家863计划(2008AA101009-7)
