摘要
试验选用体重(12.6±0.7)kg的健康莱芜猪、鲁莱和大约克夏仔猪各12头,每个品种随机分对照组和试验组,每组6头。试验组腹膜注射200μg/kg体重的脂多糖(LPS),对照组注射等量生理盐水。于注射前、后采血,测定血浆中白介素-1β(IL-1β)、皮质醇和生长激素(GH)含量。结果表明:用LPS刺激后2、4h和7h每个品种的试验组与对照组相比,血浆中IL-1β和皮质醇含量均显著提高(P<0.05),GH含量均降低,且LPS刺激后2h的GH水平显著降低(P<0.05)。3个品种猪相比,莱芜猪在LPS刺激后2h时IL-1β、皮质醇的升高幅度和GH降低幅度均最小,与大约克夏猪相比差异均显著(P<0.05)。结果显示,用LPS进行免疫应激,不同品种猪抗应激能力不同,莱芜猪相对于引进品种、地方品种有较强的抗免疫应激能力。
A total of 12 piglets （Laiwu pig and Lulai pig and Yorkshire） with （12.6＋0.7）kg live weight were divided randomly into two group, test group（6 piglets） and control group（6 piglets）. The test group of each breeds were injected i. p. with 200 g/kg BW of E. coli lipopolysaccharide （LPS）, while control group piglets were injected with equivalent sterile saline. Experimental piglets＇ blood samples were collected at pre-injection and post-injection for analysis of interleukin-1β （IL-1β）, cortisol and growth hormone （GH）. The results showed that, after LPS injection, the level of IL-1β and cortisol at 2, 4 and 7 h of test group were significantly higher than those of control group, but the level of GH was decreased, especially at 2 h after LPS injection （P 〈 0.05 ）. By the stress of LPS injection, the level of IL-1β and cortisol in blood plasma were increased and reached to the crest value at 2 h post-injection, the level of GH was decreased and reached to the lowest point at 2 h post-injection. The amplitude of change about IL-1β, cortisol and GH of Laiwu piglets were significantly smaller than that of Yorkshire（P 〈 0.05）. It indicated that different breed piglets had different ability of resistance stress. Compared to introduced variety, local variety （such as Laiwu pigs） had stronger in resistance to immunological stress.
出处
《中国畜牧杂志》
CAS
北大核心
2010年第7期19-22,共4页
Chinese Journal of Animal Science
基金
山东省良种工程重大课题项目资助(2008LZ014-04)
关键词
免疫应激
白介素-1Β
皮质醇
生长激素
仔猪
immunological stress
interleukin-1β
cortisol
growth hormone
piglets
