摘要
目的:观察pEGFP-C3-PENK/NIH3T3表达的脑啡肽对神经细胞疼痛模型兴奋性的抑制作用。方法:取新生大鼠的皮质神经元细胞培养,以106mL-1的密度种植于培养皿中。细胞分为4组:对照组(神经细胞)、前列腺素刺激组(神经细胞+42 ng/L前列腺素)、脑啡肽组(神经细胞+42 ng/L前列腺素+pEGF-C3-PENK/NIH3T3细胞)和纳洛酮拮抗组(10 mmol/L钠洛酮+神经细胞+42 ng/L前列腺素+pEGF-C3-PENK/NIH3T3细胞)。继续培养24 h后收集神经元细胞,提取总蛋白,采用Western blot测定各组神经元细胞中蛋白激酶A(PKA)的量。结果:4组细胞PKA的量比较,差异有统计学意义(F=59.873,P<0.001)。与对照组相比,前列腺素刺激组PKA增多(P<0.05);与前列腺素刺激组相比,脑啡肽组PKA减少(P<0.05);与脑啡肽组相比,纳洛酮拮抗组PKA增多(P<0.05)。结论:pEGFP-C3-PENK/NIH3T3表达的脑啡肽可以抑制神经细胞PKA系统的活化,其抑制作用可被纳洛酮拮抗。
Aim ： To observe the inhibition of excitability of nerve cell pain model by enkephalin expressing from pEG- FP-C3-PENK/NIH3T3 cells. Methods：The neonatal rat cortical neurons were cultured. Planted neurons were cultured in plates of 106 mL-1 density, then divided into four groups ： control group, prostaglandin （PG） stimulation group,PG stimula- tion plus enkephalin group,and naloxone antagonist group. Neuron cells were collected after 24 h, total protein was extrac- ted, and the amount of phosphor-protein kinase A（PKA） of each group of neurons was detected by Western blot method. ResultS：There were significant differences among four groups in PKA content（ F = 59. 873, P 〈 O. 001 ）. Compared with control group, PKA content increased in PC stimulation group （P 〈 0.05 ）. Compared with PG stimulation group, PKA con- tent reduced in PG stimulation plus enkephalin group （ P 〈 0.05 ）. Compared with PG stimulation plus enkephalin group, PKA content increased in naloxone antagonist group （P 〈 0.05 ）. Conclusion： Enkcphalin expressed by pEGFP-C3-PENK/ NIH3T3 cells could inhibit activation of PKA system of nerve cells,and the effect could be antagonisted by naloxone.
出处
《郑州大学学报（医学版）》
CAS
北大核心
2010年第2期269-272,共4页
Journal of Zhengzhou University(Medical Sciences)
