摘要
目的研究不同时间和剂量使用甲基强的松龙对急性脊髓损伤大鼠氧自由基的影响。方法利用Allen重物打击模型,选用SD大鼠60只,其中54只SD大鼠按析因设计随机分为9组,甲基强的松龙的使用剂量分别为15mg/kg、30mg/kg、60mg/kg;使用时间分别在脊髓打击前15min、30min、60min。另6只为对照组。在脊髓损伤后72h,分别进行血清、脊髓损伤段脊髓的超氧化物歧化酶(SOD)、丙二醛(MDA)的检测。结果预防性使用甲基强的松龙30mg/kg及部分60mg/kg组SOD明显升高,MDA明显降低;预防性使用甲基强的松龙的使用时间则影响不大;但提前30min使用效果最明显。结论30mg/kg、60mg/kg的甲基强的松龙对脊髓损伤大鼠SOD、MDA有影响,但以30mg/kg效果明显。其中,30mg/kg体重的甲基强的松龙在脊髓损伤前30min静脉推注对脊髓损伤大鼠氧自由基效果最好。
Objective To find out the better time and dose of administration of high-dose methylprednisolone in prophylaxis in acute spinal cord injury rats. Methods Fifty-four healthy SD rats were randomly divided into nine groups according to factor analysis. Dosages of methylprednisolone: 15 mg/kg、30 mg/kg、60 mg/kg; timing of administration: 15 min、30 min、60 min. Allen′s weight drop model of acute spinal cord injury was prepared. Methylprednisolone was administrated through caudal vein before beating as formerly described. SOD and MDA at 72-hour after SCI were respectively studied. Results MP groups of 30 mg/kg and most of 60 mg/kg showed significantly neurological effects on SCI according to SOD and MDA. And it showed that administration at 30min before SCI had been best. Conclusion MP groups of 30 mg/kg and 60 mg/kg had significantly neurological effects on SCI and MP group of 30 mg/kg was better. Administration of 30 mg/kg MP at 30min before SCI had been best among these groups.
出处
《实用临床医药杂志》
CAS
2010年第3期37-40,共4页
Journal of Clinical Medicine in Practice
基金
江苏省扬州市社会发展指导计划资助项目(YZ2005Z016)
