摘要
以水稻Oryzasativa L.等6种植物和油菜菌核病Sclerotinia sclerotiorum(Lib.)de Bary等4种植物病原菌为受试材料,采用生物测定的方法观察了青葙Celosia argenteas Linn.根氯仿提取物对植物的生物活性及抑菌作用。结果表明:与对照相比,在质量浓度为250～4000μg/mL时,提取物对水稻种子萌发的影响不显著,在4000μg/mL时,其相对抑制率仅为3.33%;在相同浓度范围内,提取物对丁香蓼Ludwigia prostrata Roxb.种子的萌发、烟草Nicotiana tabacum L.、醴肠Eclipta prostrateL.和丁香蓼幼苗的根长、水稻和三叶鬼针草Bidens pilosaL.幼苗的苗高均有显著抑制作用;对6种植物相对应的其它生长特性方面,只有在质量浓度高于250μg/mL时才受到显著抑制;在质量浓度为250～4000μg/mL时,提取物对小麦赤霉病Fusariumgra-minearum Schw.病菌的生长无显著抑制作用,在4000μg/mL时,其抑制率仅为10.30%;在相同的浓度范围内,提取物能显著抑制番茄早疫病Alternariasolani Jones et Grout.病菌的生长,抑制率为23.63%～61.01%;只有在质量浓度大于1000μg/mL时,提取物才能显著抑制油菜菌核病和杨树溃疡病Dothiorella gregariaSacc.病菌的生长。
The phytoactivity of chloroform extracts from Celosia argenteas Linn.root was measured by bioassay on testees including Oryza sativa L.,Nicotiana tabacum L.,Bidens pilosa L.,Eclipta prostrate L.,Ludwigia prostrata Roxb.,Leptochloa chinensis （Linn.） Nees and antifungal activity on Sclerotinia sclerotiovum （Lib.） de Bary,Alternaria solani Jones et Grout.,Fusarium graminearum Schw.,Dothiorella gregaria Sacc.was also measured.The results suggested that the extracts did not significantly inhibit the seed germination of O.sativa at 250～4 000 μg/mL,but significantly inhibited seed germination of L.prostrata,seedling root length of N.tabacum,E.prostrate,L.prostrata,and seedling height of O.sativa,B.pilosa.Otyer relative other traits of these six plants were significantly inhibited only over 250 μg/mL.The extracts did not significantly inhibit the growth of F.graminearum at 250～4 000 μg/mL,but significantly inhibited the growth of A.solani with an inhibition rate from 23.63% to 61.01%.The growth of S.sclerotiovum and D.gregaria was significantly inhibited only over 1 000 μg/mL.It could be concluded that chloroform extracts from C.argenteas root had the potential to be developed as bio-pesticides.
出处
《华中农业大学学报》
CAS
CSCD
北大核心
2010年第2期143-147,共5页
Journal of Huazhong Agricultural University
基金
江西省教育厅科技计划项目(GJJ09592)
井冈山大学博士科研启动项目资助
关键词
青葙
氯仿提取物
抑菌活性
Celosia argenteas Linn.
chloroform extracts
antifungal activity
