摘要
目的观察死亡相关蛋白激酶（DAPK）在甲状腺乳头状癌（PTC）中的表达，探讨DAPK在甲状腺乳头状癌发生发展中的作用。方法采用免疫组织化学SP法和Westernblot免疫印迹法检测甲状腺乳头状癌42例、癌旁组织42例（正常甲状腺组织21例和结节性甲状腺肿21例）。免疫组织化学SP法检测蛋白表达位置，Western检测蛋白表达的差异。结果免疫组织化学结果显示DAPK基因表达于细胞质及胞膜，癌旁组织中表达率73．81％（31／42）高于癌组织40．48％（17／42）。Western结果显示甲状腺乳头状癌组织中DAPK阳性表达率为35．71％（15／42），癌旁组织中为66．67％（28／42），癌旁组织的表达显著高于癌组织（χ2=8．052，P〈0．01）。淋巴结癌转移阳性的癌组织DAPK阳性表达率17．65％（3／17）低于淋巴结转移阴性的病例48．00％（12／25），（χ2=4．061，P〈0．05）。DAPK的表达与患者的年龄、性别、肿瘤大小情况无明显相关（P〉0．05）。结论DAPK参与细胞凋亡，其低表达或不表达可能参与了甲状腺乳头状癌的发生发展过程。
Objective To observe the expression of death-associated protein kinase （DAPK） in papillary thyroid carcinoma （PTC） , and explore the role of DAPK in the occurrence and development of PTC. Methods The expression of DAPK was detected by SP immunohistochemical technique and Western blotting in 42 cases of PTC tissues, 42 cases of para-PTC tissues （21 cases of normal thyroid tissues and 21 cases of nodular goiter tissues）. The location of DAPK protein expression was examined by SP immunohistochemical technique, and that the differential expression of DAPK protein by Westem blotting, respectively. Results By SP immunohistochemical technique, DAPK gene was expressed in the cytoplasm and membrane, and the positive rate of DAPK in para-PTC was 73.81% （31/42）, higher than 40.48% （17/42） in PTC. By Western blotting, the positive rate of DAPK in PTC and para-PTC tissues was 35.71% and 66.67% respectively. The positive rate of DAPK in benign thyroid issues was obviously higher than that in tumor tissues （X2 = 8. 052, P 〈 0.01 ）. The positive rate of DAPK expression in the patients with lymph node involvement was 17.65% （3/17）, lower than in those without lymph node involvement [48.00% （12/25） ] , （χ2 = 4. 061,P 〈 0.05 ）. The expression of DAPK was not associated with the age, sex, or tumor size of the patients obviously （ P 〉 0.05 ）. Conclusion DAPK is involved in cell apoptosis, and its low or even no expression may be involved in the oncogenesis of PTC.
出处
《中华实验外科杂志》
CAS
CSCD
北大核心
2010年第3期342-344,共3页
Chinese Journal of Experimental Surgery
关键词
甲状腺癌
死亡相关蛋白激酶
脱噬作用
Thyroid carcinoma
Death-associated protein kinase
Apoptosis
