摘要
目的:研究甲钴胺对周围神经再生的影响。方法:将30只Wistar大鼠随机分成对照组(N)、模型组(M)和甲钴胺组(B),每组10只。通过钳夹损伤复制大鼠坐骨神经损伤模型,对照组、模型组给予灌胃生理盐水,甲钴胺组灌胃甲钴胺水溶液,14d后观察神经纤维的数量和排列、神经传导速度、坐骨神经功能指数、后肢拉力、腓肠肌肌细胞横截面积和腓肠肌湿质量指数等指标。结果:模型组神经纤维稀疏,排列紊乱,神经传导速度减慢,坐骨神经功能指数、腓肠肌肌细胞横截面积、腓肠肌湿质量指数减小,健侧和患侧后肢拉力差值增加,与对照组比较差别有统计学意义(P<0.01);甲钴胺组神经纤维的数量较多、排列较规则,神经传导速度、坐骨神经功能指数、腓肠肌肌细胞横截面积、腓肠肌湿质量指数高于模型组(P<0.01),但低于正常组(P<0.01),后肢拉力差值低于模型组(P<0.01),但仍高于对照组(P<0.01)。结论:甲钴胺可以加快受损伤轴突的生长速度,延缓失神经支配肌肉的萎缩,促进周围神经损伤后的功能重建。
Objective： To investigate the effect of methycobal on peripheral nerve regeneration. Methods： Thirty Wistar rats were divided into normal group（N）, model group（M） and methycobal group （B）.Each group contained 10 rats. The model of rat with sciatic nerve half injury was made by clamp. Rats were fed with methycobal in B group, and same dose of normal saline was fed in N and M groups. The nerve tissue （silver nitrate stain） and gastrocnemius tissue （HE stain） were obtained to observe the morphologic index, functional index（sciatic nerve function index, differential value of hind legs tensile force, gastrocnemius wet weight index） after 14 days. Results： There were nerve fiber sparseness, disposition disorder, and decrease of gastrocne- mius cross section area, sciatic nerve function index, and differential value of hind legs tensile force, gastrocnemius wet weight index diminutu in M group compared with those of N group （P 0.01）. There were more nerve fiber, normal gastrocnemius cross section area, and higher sciatic nerve function index and gastrocnemius wet weight index in B group compared with those of M group （P 0.01）, whereas which were also different compared with those of N group （P 0.01）. Conclusion： Methycobal can promote the axon growth, postpone sarcous analosis of denervation, and enhance functional recovery of peripheral nerve injury.
出处
《天津医药》
CAS
北大核心
2010年第3期223-225,共3页
Tianjin Medical Journal
