摘要
目的建立HPLC法测定射干抗病毒注射液中绿原酸和咖啡酸的含量。方法采用Hypersil ODS柱,0.01mol·L^-1磷酸二氢钠溶液（1%磷酸溶液调节pH值至3.9）-甲醇（85∶15）为流动相,流速：1.0mL·min^-1,检测波长：323nm,柱温：30℃。结果绿原酸和咖啡酸浓度分别在0.40-200mg·L^-1（r=0.9996）、0.16-160mg.L-1（r=0.9986）范围内与峰面积呈良好线性关系,平均加样回收率分别为99.8%,100.7%。结论本法简便、准确、重复性好,可用于射干抗病毒注射液中绿原酸和咖啡酸含量的同时测定。
Objective A HPLC method for determination of chlorogenic acid and caffeic acid in Shegan Kangbingdu Injection was established.Methods A Hypersil ODS column was used with 0.01mol·L-1 sodium dihydrogen phosphate（adjust pH value to 3.9 with 1% phosphate acid solution） and methal（85∶15） as mobile phase at flow rate of 1.0mL·min^-1.The detection wavelength was 323nm and the column temperature was 30℃.Results The linear ranges of chlorogenic acid and caffeic acid were 0.40～200mg·L^-1（r=0.9996） and 0.16～160mg·L^-1（r=0.9986）,respectively.The average recoveries were 99.8% and 100.7%,respectively.Conclusion The method is simple,accurate and reproducible for determination of chlorogenic acid and caffeic acid in Shegan Kangbingdu Injection simutaneously.
出处
《中国药事》
CAS
2010年第4期386-388,共3页
Chinese Pharmaceutical Affairs
