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桔青霉CR-2内切葡聚糖酶蛋白纯化研究初探 被引量：1

Initial Studies on Endoglucanase Purification from Penicillium Citrinum Strain CR-2
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摘要 以高效纤维素分解菌桔青霉(Penicillium citrinum)CR-2菌株为材料,利用分子筛及离子交换技术纯化分离其内切葡聚糖酶蛋白,首次获得了电泳纯的桔青霉内切葡聚糖酶蛋白组分,大小为27.26kDa,最适作用温度为60℃,最适作用pH为5。对研究桔青霉纤维素酶降解纤维素的作用机理以及该菌株的改造应用具有重要意义。 Endoglucanase was purified firstly with molecular sieve and ion exchange technology from Penicillium citrinum strain CR-2.The electrophoretically purified enzyme protein was obtained.Its molecular size is 27.26 kDa.The optimum functional temperature of enzyme is 60℃ and the optimum pH is 5.This study is of great significance to explore the enzymatic degradation mechanism of cellulose by Penicillium citrinum strains and the application of CR-2 strain.
作者 顿宝庆 曲小爽 郭明鸣 路明 李桂英 张保明
机构地区 中国农业科学院作物科学研究所
出处 《中国农业科技导报》 CAS CSCD 2010年第2期98-102,共5页 Journal of Agricultural Science and Technology
基金 “十一五”国家科技支撑计划项目(2007BAD41B02) 农业部农村能源综合建设项目(2130138-018) 中国农业科学院作物科学研究所中央级公益性科研院所基本科研业务费专项资助项目(2060302-13)资助
关键词 桔青霉 内切葡聚糖酶 纯化 Penicillium citrinum endoglucanase purification
分类号 S216.3 [农业科学—农业机械化工程]
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参考文献10

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二级参考文献29

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同被引文献28

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中国农业科技导报
2010年 第2期

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