摘要
目的分析支气管哮喘(简称哮喘)儿童急性发作期及缓解期诱导痰液T细胞亚群及自然杀伤T细胞(NKT细胞)的变化,探讨T细胞及NKT细胞在儿童哮喘气道炎症中的作用。方法选取18例哮喘急性发作、21例哮喘缓解期及12名正常儿童的痰液,采用流式细胞术比较各组儿童诱导痰液CD3、CD4、CD8、NKT(CD3+CD56+)百分比及CD4/CD8比值。结果哮喘急性发作组CD4细胞百分比[(43.75±13.5)%]明显高于缓解期组[(37.04±7.11)%]和正常对照组[(33.57±7.54)%](P<0.05),CD8细胞百分比[(21.10±6.10)%]明显低于缓解期组[(28.67±5.32)%]和正常对照组[(28.31±9.46)%](P<0.05),CD4/CD8比值(2.14±0.94)高于缓解期组(1.33±0.35)和正常对照组(1.31±0.42)(P<0.05),CD4、CD8细胞百分比及CD4/CD8缓解期组和正常对照组差异无统计学意义(P>0.05),急性发作组CD4/CD8比值与嗜酸粒细胞百分比呈正相关(r=0.559,P<0.05)。缓解期组CD4/CD8比值与嗜酸粒细胞无相关性(r=0.398,P>0.05)。急性发作组CD3+CD56+细胞百分比[(3.33±1.69)%]略高于缓解组[(3.09±1.23)%]及对照组[(2.94±0.87)%],但差异无统计学意义(P>0.05)。CD3-CD56+细胞百分比在三组之间无统计学差异(P>0.05)。结论儿童哮喘急性发作期,气道内CD4细胞占优势,CD4/CD8细胞比例失衡,CD4细胞可能介导儿童哮喘气道炎症过程。
Objective To study the role of T lymphoeytes and NKT cells in air inflammation of asthmatic children, we analysis the induced sputum T lymphocyte subsets and NET cells characteristics of children with acute exacerbation asthma and remission. Methods Selected 18 cases of acute exacerbation of asthma,21 cases of asthma in remission stage and 12 cases of heahhy control children of the sputum, Flowcytometry was performed using immune cells o（ the sputum and monoclonal antibodies to CD3 ,CD4, CD8,NKT（CD3^＋CD56^＋ ）and CD4/CD8. Results The percentages of CD4 T cells[（43.75± 13.5）%] were increased in sputum of acute exacerbation asthma compared with remission asthmatics [（37.04 ± 7.11）%] and healthy controls [（ 33. 57 ± 7.54）%] （ P 〈 0.05）. The percentages of CD8^＋ T cells[（21.10± 6.10）%] in sputum were decreased in acute exacerbation asthmatic group（ P 〈0.05）. The percentage of CD4^＋ ceils in the sputum of acute exacerbation asthmatic patients was elevated resulting in a higher CD4^＋/CD8^＋ T-cell ratio（2.14±0.94） compared to the sputum of remission asthmatics（1.33±0.35） and healthy controls（1.31 ±0.42）（ P 〈0.05）. There is no differences in remission group and heahhy control group. The sputum CD4/CD8 ratio in acute exacerba tion asthmatic group correlate to the percentages of eosinophils（ r =0. 559, P 〈0.05）, there is no correlation of the CD4/CD8 ratio and the percentages of eosinophils in remission asthmatics group. Conclusions CD4^＋ T-cells were the predominant subtype of lymphocytes in children with acute exacerbation asthma, the presence of a chronic inflammation may be mediated by CD4^＋ cells in children asthma.
出处
《中华哮喘杂志（电子版）》
CAS
2010年第1期24-27,共4页
Chinese Journal of Asthma(Electronic Version)
