摘要
目的:比较不同修复方法对猪肝脏HBsAg/HBcAg免疫组化结果的影响。方法:选取15例血清HBV阳性猪肝脏组织,分别予以不修复、单纯加热高压修复和柠檬酸加热高压修复,进行免疫组化,分别检测结果。结果:免疫组化结果,不修复组阳性2例(13.3%),单纯加热高压组阳性9例(60%),柠檬酸加热高压组阳性12例(80%)。各组间差异有统计学意义(P<0.01),修复组与不修复组差异有统计学意义(P<0.01),单纯修复组和柠檬酸修复组差异无统计学意义(P>0.05)。结论:应用抗原修复能使猪肝脏HBsAg/HBcAg抗原得到较好暴露,且只需简单的加热高压即可。
Objective： To compare different retrieval methods of Swine liver HBsAg / HBcAg immunohistochemical results. Methods： 15 cases of HBV seropositive swine liver were enrolled, unrepaired and simply heated for high pressure heating repair and rehabilitation of citric acid for immunohistoehemistry was used to detect the results respectively. Results： There were 2 cases in the unrepaired group （13.2%）, 9 cases in the simple heating high-pressure group （60%）, 12 cases in the citric acid heated high- pressure group（80%）. The results of various groups were of statistically significance, （P〈0.01）,and the same was true of the results of the repair group and non-repair group, But the group of simple repair and repair of citric acid were of no significant difference （P〉 0.05）. Conclusion： Application of antigen retrieval can get a better epitope exposure of swine liver HBsAg/HBeAg, under simply heated high pressure.
出处
《大理学院学报（综合版）》
CAS
2010年第2期19-21,共3页
Journal of Dali University
基金
云南省中青年学术技术带头人后备人才培养基金资助项目(2006py01-57)
关键词
抗原
抗原修复
免疫组化
antigen
epitope retrieval
immunohistochemistry
