摘要
对新城疫病毒（ＮＤＶ）中国标准强毒Ｆ４８Ｅ８株融合蛋白的结构进行了分析．该融合蛋白的前体Ｆ０由５５３个氨基酸组成，在第１１６和１１７位氨基酸处被酶切成Ｆ１和Ｆ２亚单位．酶切激活部位序列为ＲＲＱＲＲ↓Ｆ，第１１７位氨基酸为Ｆ，具有ＮＤＶ强毒的特征．Ｆ０上有３个疏水区，其功能分别代表信号肽区、融合诱导区和跨膜结构区．Ｆ０的可糖基化部位有６个．并比较了Ｆ４８Ｅ８株和国外强毒株的氨基酸同源性，与Ｍｉｙａｄｅｒａ株、ＴｅｘａｓＧＢ株的同源性分别为９３．６４％和９２．４１％．
The amino acid sequence of the fusion (F) protein of Chinese virulent strain F 48 E 8 of Newcastle disease virus (NDV) was determined. The sequence involved a polypeptide of 553 amino acids which had essential features previously established for the F protein of virulent NDV strains. These included the presence of three strongly hydrophobic regions and the structure of Arg Arg Gln Arg Arg in the cleavage activation site. A comparison was made between amino acid sequence of the F protein of the F 48 E 8 strain and previously published sequences of the Miyadera strain and the Texas GB strain, the homology of 93.64% and 92.41% was observed respectively.
出处
《扬州大学学报（自然科学版）》
CAS
CSCD
1998年第2期31-32,共2页
Journal of Yangzhou University：Natural Science Edition
基金
江苏省自然科学基金
江苏省"九五"攻关项目
