摘要
目的:探讨荞麦糠皮提取物(EBC)对糖尿病大鼠肾损伤的抑制作用及其机制。方法:腹腔注射四氧嘧啶制成糖尿病大鼠。观察正常对照组、模型组、阳性药物组(BNPL)、低剂量EBC组、高剂量EBC组大鼠的空腹血糖(FBG)、24h尿蛋白、内生肌酐清除率(Ccr)、肾指数、肾组织果糖胺和糖基化终产物(AGEs)等指标,并通过HE和Masson染色观察肾组织形态学改变。结果:与正常对照组比,模型组FBG、24h尿蛋白、肾指数、肾组织果糖胺和AGEs含量明显升高(P<0.01),Ccr明显降低(P<0.01),且肾损伤较明显。而与模型组比,BNPL组、低剂量EBC组、高剂量EBC组均能降低FBG、24h尿蛋白、肾指数、肾组织果糖胺和AGEs含量,增加Ccr(P<0.05,P<0.01),且肾损伤程度较轻。结论:EBC对四氧嘧啶诱发糖尿病大鼠肾损伤有明显抑制作用,其机制可能是通过降低血糖,抑制AGEs形成从而减少肾损伤。
Objective：To explore inhibitory effect and its mechanism of ethanol extract from buckwheat chaff（EBC） on renal injury of diabetic rats.Methods：The diabetic rat model was induced by intraperitoneal injection with alloxan.The level of fasting blood glucose（FBG）,24-hour urine protein,creatinine clearance rate（Ccr）,renal index and fructosamine and AGEs of kidney were observed in the normal control group,model group,positive control group（administered benazepril BNPL） and low-and high-dose EBC groups.The morphological changes of kidney were observed through HE and Masson staining.Results：Compared with the normal control group,the levels of FBG,24-hour urine protein,renal index,fructosamine and AGEs of kidney in the model group exhibited a significant increase,but the level of Ccr exhibited a significant decrease（P〈0.01）.Meanwhile,obvious renal damages in the model group were observed.However,compared with the model group,the levels of FBG,24-hour urine protein,renal index and fructosamine and AGEs of kidney in BNPL treatment group and EBC treatment groups exhibited a reduction but the level of Ccr revealed an enhancement（P〈0.05,P〈0.01）,and a slight renal damage was observed in all these treatment groups.Conclusion：EBC significantly inhibits renal injury of diabetic rats induced by alloxan,and the mechanism may be related to inhibition of the formation of AGEs resulting from lowered blood glucose level.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2010年第7期276-279,共4页
Food Science
基金
河北省教育厅科技攻关项目(2006320)
唐山市"十一五"重点工程项目(072631B-1)
关键词
荞麦糠皮提取物
糖尿病
肾损伤
ethanol extract from buckwheat chaff
diabetes mellitus
renal injury
