摘要
在单因素试验的基础上,通过中心组合旋转设计与响应面分析法优化酸性丙酮法提取血红素的工艺,考察浸提时间、酸酮比以及酸性丙酮与血红蛋白的比对血红素得率的影响,并通过响应面分析法得出最佳提取条件。结果表明:最佳提取工艺条件为浸提时间42min、酸酮比3%、酸性丙酮与血红蛋白体积比5.66:1,在此条件下血红素的得率为0.225g/100mL,与预测值0.230g/100mL相差不显著。
Acetone acidified with hydrochloric acid was used as extraction solvent to extract heme from porcine red blood cells separated from whole porcine blood anticoagulated with sodium citrate by centrifugation.Single factor method and central composite design combined with response surface analysis were employed to optimize parameters such as extraction time,hydrochloric acid/acetone ratio（V/V） and acidified acetone/porcine red blood cells ratio（V/V） affecting heme extraction.Results showed that the optimal extraction for 42 min using a mixture of hydrochloric acid and acetone（3：100） at an acidified acetone/porcine red blood cells ratio of 5.66：1 gave an actual value of heme yield of 0.225 g/100 mL of whole blood,which was very close to the predicted value of 0.230 g/100 mL of whole blood.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2010年第8期60-64,共5页
Food Science
基金
湖北省重点科技攻关计划项目(2006AA206A04)
关键词
血红素
酸性丙酮法
中心组合旋转设计
响应曲面法
heme
acidified acetone method
central composite design
response surface methodology
