摘要
目的:研究小肠黏膜下层(SIS)移植替代损伤的双侧海绵体神经(CN)恢复大鼠的勃起功能。方法:制备SIS,建立动物模型,分为CN损伤组、假手术组、SIS移植组,分别给予切断双侧的CN、仅游离CN以及SIS移植修复损伤的CN。术后3个月进行阿朴吗啡试验,了解阴茎勃起情况。取中、后段阴茎海绵体组织,进行nNOS免疫组化染色,记录nNOS阳性神经纤维的数目。结果:阿朴吗啡试验:30 min内SIS移植组72.73%(8/11)的大鼠出现阴茎勃起,平均勃起(1.07±0.89)次;CN损伤组勃起率和勃起次数均为0;假手术组则为90.91%(10/11)和(2.19±1.17)次。无论是勃起率还是勃起次数,SIS移植组均显著高于CN损伤组(P<0.01),但仍然比假手术组低(P<0.05)。nNOS神经纤维数目:SIS移植组为(70.36±10.09)条,CN损伤组为(22.09±4.76)条,差异有统计学意义(P<0.01),但二者均低于假手术组[(90.81±5.69)条,P<0.01]。结论:SIS作为移植物修复损伤的大鼠CN损伤,有利于恢复CN损伤所致的勃起功能障碍。
Objective：To investigate the restoration of erectile function by reconstructing cavernous nerves（CN） with small intestinal submucosa（SIS） grafts.Methods：We prepared SIS grafts,established rat models and divided the models into a CN ablation, a sham-operation and an SIS graft group.The CNs at both sides were severed with 1 cm ablated in the first group,and 0.5 cm removed in the third,followed by reconstruction with the SIS grafts.Three months after surgery,the apomorphine test was performed to evaluate the erectile function,and then all the rats were sacrificed to detect the expression of nNOS in the penis.Results：Penile erection was observed in 72.73%（8/11） of the rats for（1.07±0.89） times within 30 min in the SIS graft group,as compared with 0%（0/11） of the rats for（0.00±0.00） times in the CN ablation group（P〈0.01）,and 90.91%（10/11） of the rats for （2.19±1.17） times in the sham-operation group（P〈0.01）.The number of nNOS nerve fibers was significantly larger in the SIS graft than in the CN ablation group（70.36±10.09 versus 22.09±4.76,P〈0.01）,but both were significantly smaller than that of the sham-operation group（90.81±5.69,P〈0.01）.Conclusion：The SIS grafting technique contributes to the recanalization of the severed CN and restoration of erectile function in rats after surgical injury.
出处
《中华男科学杂志》
CAS
CSCD
北大核心
2010年第2期150-153,共4页
National Journal of Andrology
关键词
海绵体神经
小肠黏膜下层
勃起功能
一氧化氮合酶
大鼠
cavernous nerve
small intestinal submucosa
erectile function
neuronal nitric oxide synthase
rat
