摘要
用0~3.0mg/L浓度GeO2对水稻种子浸种处理之后,在木村B培养液中培养,并分别以6个浓度继续添加GeO2溶液.另用不同浓度的GeO2和SiO2混合溶液培养水稻,测定其营养生长期的整株鲜重、根鲜重、地上部高、叶绿素含量、根系活力以及叶过氧化氢酶(CAT)、叶多酚氧化酶(PPO)、根过氧化物酶(根PO)和叶(PO).实验表明,随着培养时间延长,上述生理指标值有所变化,其中前6项正常生理指标值,随着GeO2浓度提高而减少,后3项衰老指标则随着GeO2浓度提高而有增加趋势,表明3种酶活性的提高以缓解GeO2对水稻的毒害作用.各生理指标与GeO2浓度之间呈明显相关关系.同时,还发现了一定浓度的GeO2对水稻生长有抑制和毒害作用,而较高浓度的SiO2可适度缓解该抑制作用.
Affer soaking in the GeO 2 solution of 0 ̄3.0 mg/L, some rice seeds are cultured in the solutions with six diffrent concentration of GeO 2 and SiO 2. The fresh weight of all plant, fresh weight of the root stock, plant height, chlorophyll contain; activity of the root, cetalase activity of teaf, polyphenoloxidase of root, and perosidase of root and leaf were studied during the growth and development period. The results shows that these nine physiological indexes are increasing with the culture going on. The former six indexes decrease with increasing the concentration of GeO 2 in culture. but the latter three aged indexes increase with increasing the concentration of GeO 2, indicating that the activity of these three kinds of enzyme are enhancing to defy the inhibition of GeO 2 to a certain degree. At the same time, high concentration of SiO 2 is thought to be a way to ease the inhibition of GeO 2.
出处
《厦门大学学报(自然科学版)》
CAS
CSCD
北大核心
1999年第1期121-128,共8页
Journal of Xiamen University:Natural Science
关键词
营养生长
酶活性
生理指标
水稻
锗
硅
Growth and development, Enzyme activity, Physiological indexes, Ge Si antagostic action