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人乳头瘤病毒核酸检测技术问题探讨 被引量：5

Technical issues of HPV-DNA detection
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摘要 人乳头瘤病毒(HPV)的持续感染是导致宫颈癌发生的重要因素。该文详细列举了HPV核酸检测的技术和方法 ,包括DNA印迹杂交、原位杂交、聚合酶链反应(PCR)、杂交捕获试验(HC)、低密度基因芯片导流杂交技术、InvaderTechnolo-gy等,并讨论了各种检测技术的特点和存在的问题,为选择合适的HPV核酸检测方法提供依据。 human papillomavirus （HPV） infection is a necessary factor in the development of cervical cancer. This report itemizes several kinds of technology for HPV DNA test, including DNA Southern blotting, In situ hybridization, PCR, HC, Flow-through hybridization and Invader Technology respectively. Here we discussed advantages and disadvantages of these technologies, The purpose is to provide the basis for appropriate HPV DNA test technology.
作者 赵健 廖秦平
机构地区 北京大学第一医院妇产科
出处 《中国实用妇科与产科杂志》 CAS CSCD 北大核心 2010年第5期344-346,共3页 Chinese Journal of Practical Gynecology and Obstetrics
关键词 人乳头瘤病毒 聚合酶链反应 杂交捕获 导流杂交 human papillomavirns PCR hybridization capture flow-through hybridization
分类号 R71 [医药卫生—妇产科学]
  • 相关文献

参考文献7

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  • 6赵健,杨英捷,廖秦平.导流杂交基因芯片技术在人乳头状瘤病毒感染分型检测中的临床应用[J].中华检验医学杂志,2006,29(12):1148-1151. 被引量：81
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二级参考文献10

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共引文献80

同被引文献34

  • 1王彬,陈凤娴.新柏氏液基细胞学检查在宫颈病变中的应用[J].重庆医学,2006,35(13):1195-1196. 被引量：5
  • 2赵健,杨英捷,廖秦平.导流杂交基因芯片技术在人乳头状瘤病毒感染分型检测中的临床应用[J].中华检验医学杂志,2006,29(12):1148-1151. 被引量：81
  • 3卞美璐.WHO(2006年)宫颈癌综合防治实践指南简介[J].中国实用妇科与产科杂志,2007,23(7):557-560. 被引量：106
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引证文献5

二级引证文献39

中国实用妇科与产科杂志
2010年 第5期

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