摘要
研究了小蜡树枝叶中皂甙类物质的提取工艺及其抗氧化活性。应用单因素和正交试验对浸提温度、乙醇浓度、浸提时间、料液比进行了研究,同时采用邻二氮菲体系和水杨酸体系研究了小腊树枝叶皂甙清除·OH的抗氧化活性。实验得出小蜡树枝叶中总皂甙的最佳提取条件是以80%的乙醇作提取剂,1:70的料液比,温度为85℃,提取时间为2h。在最佳浸提条件下,小腊树叶中皂甙的得率为39.270mg/g,小腊树枝中皂甙的得率为22.998mg/g。在水杨酸体系中小腊树枝、叶的皂甙对羟自由基的EC50为0.937、0.720mg/mL,二者都低于Vc(EC50=0.098mg/mL)的。在邻菲啰啉体系中小腊树叶、枝皂甙、Vc对羟自由基的清除率相当,分别为0.259、0.253、0.259mg/mL。
In this paper,the extracting technology of saponin from the leaves and branches from Ligustrum quihoui Carr.were studied,as well as its antioxidation in vitro.First of all,the with single-factor experiments and orthogonal design,the extraction temperature,the ethanol concentration,extracting time,ratio of material to liquid were probed,at the same time the elimination effect on the ·OH of saponin were also studied with the Salicylic acid system and 1,10-phenanthroline-Fe2+ systme.The results were showed that the optimal extracting conditions of saponin were that using 80% ethanol for extraction agent,in accordance with the one seventy liquid ratio,temperature 85 ℃,extraction time of 2 h.at the best extraction conditions,the saponin yield from Ligustrum quihoui Carr.leaves was 39.270 mg/g,the branches'was 22.998 mg/g.In the Salicylic acid system,the EC50 of the branches and leaves of Ligustrum quihoui Carr.were 0.937 mg/mL and 0.720 mg/mL,and they were lower than the EC50 of Vc(0.098 mg/mL),and in the 1,10-phenanthroline-Fe2+ systme,the elimination effect on the hydroxyl free radical of the leaves,branches and Vc were close,the EC50 were separately 0.259,0.253,0.259 mg/mL.
出处
《食品科技》
CAS
北大核心
2010年第5期230-233,共4页
Food Science and Technology
基金
湖北民族学院青年项目
关键词
小腊树
皂甙
提取
羟自由基
Ligustrum quihoui Carr.
saponin
extraction
·OH
