摘要
目的验证焦磷酸测序法检测外周血白细胞基因多态性的可靠性,为临床快速检测患者基因型制定合理的个体化治疗方案提供依据。方法选取96例全血样标本,用焦磷酸测序法检测外周血白细胞乙醛脱氢酶(ALDH2)基因型。结果焦磷酸测序法检测ALDH2*1、ALDH2*2各等位基因频率分别为87%和13%,各基因型所占频率ALDH2*1/*1:72例(75%),ALDH2*1/*2:23例(24%),ALDH2*2/*2:1例(1%),与文献中报道结果相一致。结论焦磷酸测序法对AL-DH2基因型检测结果准确、可靠,且方便快捷,适用于临床相关药物基因快速检测。
Objective To check the reliability of pyrosequencing in detecting the polymorphism of genes from peripheral blood leucocytes,and to establish this method for rapid detection of the genotype in patients and making rational individualized treatment.Methods Pyrosequencing was used to detect the genotype of ALDH2 from 96 samples.Results The amount of ALDH2＊1/＊1、ALDH2＊1/＊2、ALDH2＊2/＊2 was 72,23 and 1 respectively by pyrosequencing,consistent with results reported.The distributed frequency of ALDH2＊1/＊1,ALDH2＊1/＊2,ALDH2＊2/＊2 was 75%,24%,1% respectively.Conclusion It is accurate,reliable and simple to detect genetic polymorphism of ALDH2 by pyrosequencing which is able to detect genes of correlated medicine quickly.
出处
《解放军药学学报》
CAS
2010年第1期1-3,共3页
Pharmaceutical Journal of Chinese People's Liberation Army
