摘要
通过正交试验得出Arazyme蛋白酶酶解地鳖虫的最佳工艺条件为加酶量300U,酶解时间5h,料水比1:20。并分析了其酶解液对羟自由基(·OH)、超氧阴离子自由基(O2-·)、二苯代苦味酰基自由基(DPPH)的清除作用以及对亚油酸自氧化反应的抑制作用。结果表明:地鳖虫酶解液对·OH、O2-·、DPPH三种自由基的最高清除率分别为75.15%(浓度为50g/L)、31.72%(浓度为2.5g/L)、74.88%(浓度为80mg/L);在肽含量为2.0mg时,对亚油酸自氧化反应的抑制率为95%。
The optimum conditions of enzymatic hydrolysis by Arazyme protease of Eupoliphaga Sinensis Walker were enayme concentration 300 U,time of hydrolysis 5 h,and solid-to-liquid ratio 1：20.The free radicals scavenging activity on·OH,O2^-·,DPPH and linoleic acid oxidation inhibition of hydrolysate was invistgated.The result showed that the highest scavenging activityof·OH,O2^-·and DPPH was 75.15 %（concentration 50 g/L）,31.72 %（concentration 2.5 g/L）,74.88 %（concentration 80 mg/L）.The linoleic acid oxidation inhibition was 95 % （concentration of peptide was 2 mg）.
出处
《食品研究与开发》
CAS
北大核心
2010年第3期159-162,166,共5页
Food Research and Development
关键词
地鳖虫
自由基
亚油酸自氧化
Eupoliphaga Sinensis Walker
free radicals
linoleic acid model system
