摘要
目的:研究三七总苷(PNS)对大鼠高脂血症性骨质疏松的作用。方法:取SPF级3月龄未交配SD大鼠30只随机分为3组,每组10只。正常对照组:普通饲料喂养,蒸馏水5mL.kg-1.d-1灌胃;模型组:脂肪乳剂5mL.kg-1.d-1灌胃;PNS组:上午予脂肪乳剂5mL.kg-1.d-1灌胃,下午予PNS200mg·kg-1.d-1灌胃,连续20周。实验结束后用骨组织形态计量学方法,测定大鼠右侧胫骨近端松质骨静态参数和动态参数,并观察血清生化指标。结果:与模型组比较,PNS组游离脂肪酸(FFA)、TC、低密度脂蛋白胆固醇(LDL-C)均明显降低(P<0.05),高密度脂蛋白胆固醇(HDL-C)明显升高(P<0.05);松质骨形态计量学静态参数骨小梁面积百分数(%Tb.Ar)、骨小梁数量(Tb.N)、骨小梁厚度(Tb.Th)均增加(P<0.01),骨小梁分离度(Tb.Sp)明显减小(P<0.01);动态参数荧光周长百分率(%L.Pm)、骨形成率(BFR/BS、BFR/BV)均明显增加(P<0.05)。结论:脂肪乳剂长期灌胃可导致大鼠脂质代谢紊乱,并伴有高脂血症性骨质疏松症。PNS可显著降低血脂并促进骨的形成,对大鼠高脂血症所致骨质疏松有一定防治作用。
AIM： To explore the effects of panax notoginsenosides （PNS） on bone histomorphometry in rats with hyperlipidemia-induced osteoporosis. METHODS.. Thirty 3-month-old Sprague-Dawley rats were randomly divided into three groups with 10 per group as follows. Control group was intragastrically given distilled water at the dose of 5 mL·kg^-1·d^-1 for 20 weeks; Model group was intragastrically given lipid emulsion at the dose of 5 mL·kg^-1·d^-1 for 20 weeks; PNS preventing group was intragastrically given lipid emulsion at the dose of 5 mL·kg^-1·d^-1 in the morning and PNS at the dose of 5 mL·kg^-1·d^-1 at afternoon for 20 weeks. At the end of the experiment, the right longitudinal proximal tibial metaphyseal parameters of rats were performed undecalcifiedly and used for bone histomorphometry analysis. And the blood serum contents of FFA, TG, TC,HDL-C and LDL-C were measured by biochemical analysis. RESULTS： Compared with model group, the blood serum contents of FFA, TC and LDL-C were decreased and HDL-C was increased significantly（P〈0.01）, the PTM cancellous bone of % Tb. Ar, Tb. Th and Tb. N were increased（P〈0.01） and Tb. Sp was de creased significantly （P〈 0.01）. The dynamic parameters in cancellous bone of % L. Pm and bone formation rate （BFR/BS, BFR/BV） were increased（P〈0.05）. CONCLUSION. Long-term gastric perfusion of lipid emulsion could induce hyperlipidemia-induced osteoporosis, and PNS could partly prevent it.
出处
《中国临床药理学与治疗学》
CAS
CSCD
2010年第3期277-281,共5页
Chinese Journal of Clinical Pharmacology and Therapeutics
关键词
三七总苷
骨质疏松
高脂血症
骨组织形态计量学
Panax notoginsenosides
Osteoporosis
Hyperlipidemia
Bone histomorphometry
