摘要
以平贝母为供试材料,研究了组培平贝母培养过程中培养体不同发育时期同工酶的变化。结果表明:在发育过程中各培养体的酯酶同工酶的酶带较丰富,共检测出11条酶带,且形成小鳞茎后酶谱的酶带数都明显多于愈伤组织和胚状体时期,且愈伤组织在Rf=0.708处产生了特异性酶带。过氧化氢酶在愈伤组织、胚状体和刚形成小鳞茎时都有表达,且酶谱仅有1条带,Rf=0.042,而在鳞茎膨大时没有该酶的表达。
Taking Fritillaria ussuriensis Maxim.as experiment material,the isozyme changes in different stage of tissue culture during culturing of Fritillaria ussuriensis Maxim.were studied.The results showed that enzyme bands of esterase isozyme were abundant and examined 11 bars;Enzyme bands were obviously more than callus and embryoid after forming small bulblet and callus presented differential band at Rf =0.708.Catalase isozyme expressed and had only one bar during calluse,embryoid and small bulblet formation,Rf was 0.042,while the catalase isozyme was not expressed in bulblet expanding.
出处
《黑龙江农业科学》
2010年第2期1-3,共3页
Heilongjiang Agricultural Sciences
基金
延边大学科技发展计划资助项目(延大科合字(2008)第05号)
关键词
平贝母
培养体
酯酶
过氧化氢酶
Fritillaria ussuriensis Maxim.
culture
esterase
catalase
