摘要
目的 探讨分离自中华按蚊雌成蚊中肠的3种革兰阴性细菌,即气单孢菌、A4菌（未鉴定）和肠杆菌在按蚊幼虫孳牛水体中及按蚊体内的存活能力. 方法 3种目标细菌在实验室经数量扩增后,添加到饲养按蚊幼虫的水体中,依次收集饲养盆和蛹盒内的水样品,并解剖羽化后吸血雌成蚊,取其中肠制成研磨液;建立水样品和研磨液中细菌的16S rDNA序列克隆文库,并从各样品中分离可培养细菌,比较各样品中的优势菌种和不同处理中可培养细菌的种类组成. 结果 在所有样品中均未检测到与加入的人工培养细菌种类相同的细菌.按蚊幼虫饲养水体和蛹盒水体中的可培养细菌均以嗜水气单孢菌和睾丸酮丛毛单胞菌为主,在少量按蚊的中肠内含有可培养细菌,但均不是目标细菌;而各处理样品中的优势菌均为铜绿假单孢菌. 结论 3种细菌经人工培养后,不能在按蚊幼虫生活水体中建立种群,导致未能在按蚊成蚊中肠内柃测到这些细菌.
Objective To investigate the survival ability of 3 species of gram-negative bacteria, Aero-monas sp. , A4 and Enterobacter sp. in water where larva anopheline mosquitoes developed from larva to adults and in midguts of adult Anopheles sinensis. Methods The 3 species of bacteria were cultivated in laboratory and added to the mosquito-rear-cups. During the period of the mosquitoes developing, water samples in mosquito-rear-cups and pupae boxes were collected when the mosquitoes finished their development in them. Midguts of adult An. sinensis samples were also collected and grinded in 0.9% NaCl solutions. Bacteria floras of cultivable bacteria and dominant bacteria were determined in all samples based on 16S rDNA sequences of cultivable bacteria and 16S rDNA cloning libraries of all samples. Bacteria floras were compared between treatments which treated with different bacteria and between different samples of same treatment. Results No target bacteria were detected in the test samples. Dominant cultivable bacteria of all treatments in water of mosquito-rear-cups and pupae boxes were A. hydrophila and Comamonas testosterone, respectively. Cultivable bacteria in midguts of female mosquitoes were also not target bacteria. Based on 16S rDNA cloning libraries, P. aeruginosa were the only dominant bacteria detected in all samples. Conclusion All 3 target bacteria cultivated in laboratory could not establish populations in mosquito-rear-cups that led to no target bacteria could be detected in the midguts of female adult mosquitoes.
出处
《国际医学寄生虫病杂志》
CAS
2010年第3期148-151,共4页
International JOurnal of Medical Parasitic Diseases
关键词
细菌菌群
优势细菌
中肠细菌
施用
经发育期传递
Bacteria flora
Dominant bacteria
Midgut bacteria
Application
Transstadial transmission
