摘要
以杂交新美柳Salix matsudana×alba幼苗为研究材料,采用LI-6400便携式光合测定系统对其光合特性进行研究。结果表明:①自然生长季节,杂交新美柳叶片的净光合速率(Pn)日变化呈单峰曲线,最高峰出现在中午11:00,其最大净光合速率(Pmax)为20.8μmol.m-2.s-1。②在控制二氧化碳摩尔分数和温度的条件下,光饱和点(PLS)为1847.6μmol.m-2.s-1,光补偿点(PLC)为58.1μmol.m-2.s-1。杂交新美柳的光饱和点和光补偿点都较高,表明它是一种阳性植物。③在控制光照强度和温度的条件下,利用Farquhar模型对杂交新美柳叶片净光合速率-胞间二氧化碳摩尔分数的响应进行拟合,当胞间二氧化碳摩尔分数小于400μmol.mol-1时,可算得其最大羧化速率(Vcmax)为91.6μmol.m-2.s-1,二氧化碳补偿点(Г*)为46.5μmol.mol-1,呼吸速率(Rd)为4.9μmol.m-2.s-1;升高二氧化碳摩尔分数可使杂交新美柳的净光合速率增大,提高叶片对光能的利用率,其叶片二氧化碳饱和点(PCS)大约在1000μmol.mol-1,同时可算得其最大电子传递速率(Jmax)为256.0μmol.m-2.s-1;当二氧化碳过饱和(>1000μmol.mol-1)时,可算得其磷酸丙糖利用速率(UTP)为19.7μmol.m-2.s-1。
The LI-6400 portable photosynthesis measuring system was used to determine photosynthetic characteristics of leaves in one-year-old seedlings of Salix matsudana × alba and then fitted to the Farquhar model. Results showed that (1) During the growing season,diurnal changes in leaf net photosynthetic rate (Pn) had a single-peak curve that appeared at 11 :00 o'clock with a maximum net photosynthetic rate (Pmax) of 20.8 μmol.m-2.s-1. (2) When CO2 concentration (350 μmol.mol-1) and temperature(25 ℃) were controlled,the photosynthetic light saturation point (PLS) was 1 847.6 μmol.m-2.s-1,and the photosynthetic light compensation point (PLC) was 58.1 μmol.m-2.s-1 indicating a sun-tolerant plant. (3) The Farquhar model fitting the response of leaf Pn to intercellular CO2 concentration(Ci),with light intensity(1 500 μmol. m-2.s-1) and temperature (25 ℃) being controlled,showed that when Ci〈400 μmol.mol-1,the maximum carboxylation rate (Vcmax) was 91.6 μmol.m-2.s-1,the CO2 compensation point (Г *) was 46.5 μmol.mol-1,and the respiration rate (Rd) was 4.9 μmol.m-2.s-1. From 400 to 1 000 μmol.mol-1 of Ci,increasing the CO2 concentration increased leaf Pn and light utilization efficiency with a leaf CO2 saturation point (PCS) of about 1 000 μmol.mol-1 and a maximum electron transport rate (Jmax) of 256.0 μmol.m-2.s-1. When Ci was supersaturated (〉1 000 μmol.mol-1),the rate of leaf triose phosphate utilization (UTP) was 19.7 μmol.m-2. s-1.
出处
《浙江林学院学报》
CSCD
北大核心
2010年第3期340-346,共7页
Journal of Zhejiang Forestry College
基金
江苏省科学技术攻关项目(BE2006342)